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Infection and Immunity, September 2003, p. 5306-5313, Vol. 71, No. 9
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.9.5306-5313.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparative Analysis of Plant and Animal Models for Characterization of Burkholderia cepacia Virulence

Steve P. Bernier,¹ Laura Silo-Suh,^2, Donald E. Woods,¹ Dennis E. Ohman,^2,3 and Pamela A. Sokol^1*

Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta T2N 4N1, Canada,¹ Department of Microbiology and Immunology, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23298-0678,² McGuire Veteran Affairs Medical Center, Richmond, Virginia 23249³

Received 26 February 2003/ Returned for modification 13 May 2003/ Accepted 26 June 2003

A simple alfalfa model was developed as an alternative infection model for virulence studies of the Burkholderia cepacia complex. Symptoms of disease were observed in wounded alfalfa seedlings within 7 days following inoculation of 10¹ to 10⁵ CFU of most strains of the B. cepacia complex. Strains from seven genomovars of the B. cepacia complex were tested for virulence in the alfalfa model, and the degree of virulence was generally similar in strains belonging to the same genomovar. Strains of Burkholderia multivorans and some strains of Burkholderia stabilis did not cause symptoms of disease in alfalfa seedlings. Representative strains were also tested for virulence using the rat agar bead model. Most of the strains tested were able to establish chronic lung infections; B. stabilis strains were the exception. Most of the strains that were virulent in the alfalfa infection model were also virulent in the lung infection model. The B. cepacia genomovar III mutants K56pvdA::tp and K56-H15 were significantly less virulent in the alfalfa infection model than their parent strain. Therefore, this alfalfa infection model may be a useful tool for assessing virulence of strains of the B. cepacia complex and identifying new virulence-associated genes.

Editor: V. J. DiRita

Present address: Department of Biological Sciences, Auburn University, Auburn, AL 36849.

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