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Infection and Immunity, September 2003, p. 5381-5385, Vol. 71, No. 9
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.9.5381-5385.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Two-Component Systems of Helicobacter pylori Contribute to Virulence in a Mouse Infection Model

Klaus Panthel,¹ Patricia Dietz,^2, Rainer Haas,¹ and Dagmar Beier^2*

Max-von-Pettenkofer Institut für Hygiene und Medizinische Mikrobiologie, D-80336 Munich,¹ Theodor-Boveri-Institut für Biowissenschaften, Lehrstuhl für Mikrobiologie, Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany²

Received 11 February 2003/ Returned for modification 28 March 2003/ Accepted 27 May 2003

Helicobacter pylori encodes three histidine kinases and five response regulators belonging to the family of two-component regulatory systems which are involved in transcriptional control. Here we demonstrate that isogenic mutants of H. pylori P76 with deletions of the response regulator open reading frame (ORF) HP1365 and ORFs HP244, HP165, and HP1364 encoding histidine kinases are unable to colonize the stomachs of BALB/c mice, suggesting an essential role of these systems in the regulation of important virulence properties of H. pylori. Furthermore, we demonstrate that the genes under the control of the P_HP1408 and P_HP119 promoters which are regulated by the two-component system HP166-HP165 are not essential for single mutant colonization of mice but are required under competitive colonization conditions.

Editor: A. D. O'Brien

Present address: BIOMED R/T, 64271 Darmstadt, Germany.

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