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Infection and Immunity, January 2004, p. 611-614, Vol. 72, No. 1
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.1.611-614.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Activation of Serratia marcescens Hemolysin through a Conformational Change

Georg Walker, Ralf Hertle, and Volkmar Braun*

Mikrobiologie/Membranphysiologie, Universität Tübingen, Tübingen, Germany

Received 28 July 2003/ Returned for modification 4 September 2003/ Accepted 25 September 2003

For Serratia marcescens, secreted hemolysin/cytotoxin is not only secreted but also activated by an outer membrane protein. Excluding posttranslational processing by mass spectrometry, the conformation of active and inactive ShlA derivatives strongly differed in electrophoretic mobilities, gel permeation chromatography, sensitivity to trypsin, circular dichroism, and intrinsic fluorescence. We concluded that ShlB interacts with ShlA during secretion and imposes a conformational change in ShlA to form the active hemolysin.

* Corresponding author. Mailing address: Mikrobiologie/Membranphysiologie, Auf der Morgenstelle 28, D-72076 Tübingen, Germany. Phone: (49) 7071 29 72096. Fax: (49) 7071 295843. E-mail: volkmar.braun{at}mikrobio.uni-tuebingen.de.

Editor: J. T. Barbieri

Infection and Immunity, January 2004, p. 611-614, Vol. 72, No. 1
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.1.611-614.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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