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Infection and Immunity, October 2004, p. 5886-5891, Vol. 72, No. 10
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.10.5886-5891.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Invasion Profiles of Brazilian Field Isolates of Plasmodium falciparum: Phenotypic and Genotypic Analyses{dagger}

Cheryl-Ann Lobo,1* Karla de Frazao,2 Marilis Rodriguez,1 Marion Reid,3 Mariano Zalis,2 and Sara Lustigman1

Molecular Parasitology,1 Immunohematology, Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York,3 Federal University of Rio de Janeiro, Rio de Janeiro, Brazil2

Received 5 April 2004/ Returned for modification 22 May 2004/ Accepted 28 June 2004

The invasion of red blood cells (RBCs) by Plasmodium falciparum is dependent on multiple molecular interactions between erythrocyte receptors and parasite ligands. Invasion studies using culture-adapted parasite strains have indicated significant receptor heterogeneity. It is not known whether this heterogeneity reflects the parasite invasion arsenal in the field. We have studied the invasion phenotypes of 14 distinct field isolates from the Legal Amazon areas of Brazil by using erythrocyte invasion assays to investigate invasion into normal, enzyme-treated, and clinical-mutant RBCs. Analysis of these isolates revealed four distinct invasion profiles. Using En(a–) cells to get an unequivocal estimate of the use of glycophorin A (GPA) as a receptor, we found that the 175-kDa erythrocyte-binding antigen (EBA-175)/GPA pathway was used by a minority of the parasite isolates studied. Although polymorphism of region II domains at specific amino acid positions in both EBA-140 and EBA-181 was found in these field isolates, this did not correlate with invasion profiles and thus receptor selectivity. These studies have further confirmed the existence of a significant diversity of invasion pathways in nature and suggest that additional parasite ligands will have to be targeted to devise global vaccines that will work in the field.

* Corresponding author. Mailing address: Molecular Parasitology, Lindsley Kimball Research Institute, New York Blood Center, 310 E. 67th St., New York, NY 10021. Phone: (212) 570-3415. Fax: (212) 570-3121. E-mail: clobo{at}nybloodcenter.org.

{dagger} This paper is dedicated to the memory of Karla de Frazao.

Editor: W. A. Petri, Jr.

Infection and Immunity, October 2004, p. 5886-5891, Vol. 72, No. 10
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.10.5886-5891.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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