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Infection and Immunity, November 2004, p. 6324-6329, Vol. 72, No. 11
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.11.6324-6329.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

RNA Encoding the MPT83 Antigen Induces Protective Immune Responses against Mycobacterium tuberculosis Infection

Tian Xue,1 Evangelos Stavropoulos,1 Min Yang,1 Silvia Ragno,1 Martin Vordermeier,2 Mark Chambers,2 Glyn Hewinson,2 Douglas B. Lowrie,1 M. Joseph Colston,1 and Ricardo E. Tascon1*

Mycobacterial Division, National Institute for Medical Research, London,1 Central Veterinary Laboratory, Addlestone, Surrey, United Kingdom2

Received 7 March 2004/ Returned for modification 3 May 2004/ Accepted 30 July 2004

We have previously demonstrated that vaccination of mice with plasmid DNA vectors expressing immunodominant mycobacterial genes induced cellular immune responses and significant protection against challenge with Mycobacterium tuberculosis. We demonstrate here, using in vitro-synthesized RNA, that vaccination with DNA or RNA constructs expressing the M. tuberculosis MPT83 antigen are capable of inducing specific humoral and T-cell immune responses and confer modest but significant protection against M. tuberculosis challenge in mice. This is the first report of protective immunity conferred against intracellular bacteria by an RNA vaccine. This novel approach avoids some of the drawbacks of DNA vaccines and illustrates the potential for developing new antimycobacterial immunization strategies.

* Corresponding author. Mailing address: Mycobacterial Division, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom. Phone: (020) 89599 3666. Fax: (020) 8913 8528. E-mail: tricard{at}nimr.mrc.ac.uk.

Editor: S. H. E. Kaufmann

Infection and Immunity, November 2004, p. 6324-6329, Vol. 72, No. 11
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.11.6324-6329.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Luo, D., Ni, B., Li, P., Shi, W., Zhang, S., Han, Y., Mao, L., He, Y., Wu, Y., Wang, X. (2006). Protective Immunity Elicited by a Divalent DNA Vaccine Encoding Both the L7/L12 and Omp16 Genes of Brucella abortus in BALB/c Mice.. Infect. Immun. 74: 2734-2741 [Abstract] [Full Text]  


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