This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kirby, A. C.
Right arrow Articles by Wick, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kirby, A. C.
Right arrow Articles by Wick, M. J.

 Previous Article  |  Next Article 

Infection and Immunity, November 2004, p. 6390-6400, Vol. 72, No. 11
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.11.6390-6400.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

In Vivo Compartmentalization of Functionally Distinct, Rapidly Responsive Antigen-Specific T-Cell Populations in DNA-Immunized or Salmonella enterica Serovar Typhimurium-Infected Mice

Alun C. Kirby,1,{dagger} Malin Sundquist,2 and Mary Jo Wick1,2*

Department of Cell and Molecular Biology, Section for Immunology, Lund University, Lund,1 Department of Clinical Immunology, Göteborg University, Göteborg, Sweden2

Received 26 August 2003/ Returned for modification 24 October 2003/ Accepted 26 July 2004

The location and functional properties of antigen-specific memory T-cell populations in lymphoid and nonlymphoid compartments following DNA immunization or infection with Salmonella were investigated. Epitope-specific CD8+-T-cell expansion and retention during the memory phase were analyzed for DNA-immunized mice by use of a 5-h peptide restimulation assay. These data revealed that epitope-specific gamma interferon (IFN-{gamma})-positive CD8+ T cells occur at higher frequencies in the spleen, liver, and blood than in draining or peripheral lymph nodes during the expansion phase. Moreover, this distribution is maintained into long-term memory. The location and function of both CD4+ and CD8+ Salmonella-specific memory T cells in mice who were given a single dose of Salmonella enterica serovar Typhimurium was also quantitated by an ex vivo restimulation with bacterial lysate to detect the total Salmonella-specific memory pool. Mice immunized up to 6 months previously with S. enterica serovar Typhimurium had bacterium-specific CD4+ T cells that were capable of producing IFN-{gamma} or tumor necrosis factor alpha (TNF-{alpha}) at each site analyzed. Similar findings were observed for CD8+ T cells that were capable of producing IFN-{gamma}, while a much lower frequency and more restricted distribution were associated with TNF-{alpha}-producing CD8+ T cells. This study is the first to assess the frequencies, locations, and functions of both CD4+ and CD8+ memory T-cell populations in the same Salmonella-infected individuals and demonstrates the organ-specific functional compartmentalization of memory T cells after Salmonella infection.

* Corresponding author. Present address: Department of Clinical Immunology, Göteborg University, Guldhedsgatan 10, SE 413 46 Göteborg, Sweden. Phone: 46 31 342 4602. Fax: 46 31 342 4621. E-mail: mary-jo.wick{at}immuno.gu.se.

Editor: S. H. E. Kaufmann

{dagger} Present address: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom.

Infection and Immunity, November 2004, p. 6390-6400, Vol. 72, No. 11
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.11.6390-6400.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Berndt, A., Wilhelm, A., Jugert, C., Pieper, J., Sachse, K., Methner, U. (2007). Chicken Cecum Immune Response to Salmonella enterica Serovars of Different Levels of Invasiveness. Infect. Immun. 75: 5993-6007 [Abstract] [Full Text]  
  • Jones-Carson, J., McCollister, B. D., Clambey, E. T., Vazquez-Torres, A. (2007). Systemic CD8 T-Cell Memory Response to a Salmonella Pathogenicity Island 2 Effector Is Restricted to Salmonella enterica Encountered in the Gastrointestinal Mucosa. Infect. Immun. 75: 2708-2716 [Abstract] [Full Text]  
  • Lindell, D. M., Ballinger, M. N., McDonald, R. A., Toews, G. B., Huffnagle, G. B. (2006). Immunologic Homeostasis during Infection: Coexistence of Strong Pulmonary Cell-Mediated Immunity to Secondary Cryptococcus neoformans Infection While the Primary Infection Still Persists at Low Levels in the Lungs. J. Immunol. 177: 4652-4661 [Abstract] [Full Text]  
  • Ravindran, R., Foley, J., Stoklasek, T., Glimcher, L. H., McSorley, S. J. (2005). Expression of T-bet by CD4 T Cells Is Essential for Resistance to Salmonella Infection. J. Immunol. 175: 4603-4610 [Abstract] [Full Text]  
  • Sundquist, M., Wick, M. J. (2005). TNF-{alpha}-Dependent and -Independent Maturation of Dendritic Cells and Recruited CD11cintCD11b+ Cells during Oral Salmonella Infection. J. Immunol. 175: 3287-3298 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»