This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gilbertson, B.
Right arrow Articles by Cheers, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gilbertson, B.
Right arrow Articles by Cheers, C.

 Previous Article  |  Next Article 

Infection and Immunity, December 2004, p. 6884-6891, Vol. 72, No. 12
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.12.6884-6891.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Bystander Activation of CD8+ T Lymphocytes during Experimental Mycobacterial Infection

Brad Gilbertson,1 Susie Germano,1 Pauline Steele,1,{dagger} Steven Turner,1 Barbara Fazekas de St. Groth,2 and Christina Cheers1*

Department of Microbiology and Immunology, University of Melbourne, Victoria,1 Centenary Institute of Cancer Medicine and Cell Biology, Newtown, New South Wales, Australia2

Received 15 June 2004/ Returned for modification 21 July 2004/ Accepted 22 August 2004

Infection of C57BL/6 mice with Mycobacterium avium leads to the activation of both CD4+ and CD8+ gamma interferon (IFN-{gamma})-producing T cells, although the CD8+ cells play no role in protection against infection. Using transfer of different lines of transgenic T cells with T-cell receptors (TCRs) which recognize irrelevant antigens, we show here that transferred CD8+ T cells from two of the three lines were activated to the same degree as the host cells, suggesting that the majority of the IFN-{gamma}-producing CD8+ T cells of the host represented bystander activation. The third line, specific for the male HY antigen, showed no activation. Activation required the participation of the CD28 coreceptor on T cells and was unaffected by the removal of CD44hi (memory phenotype) T cells. The transferred CD8+ T cells proliferated in vivo, although this was not essential for IFN-{gamma} production. Taken together, these data are highly reminiscent of homeostatic proliferation of TCR transgenic T cells upon transfer to lymphopenic hosts, and suggest low-affinity stimulation through the TCR, possibly by self peptides. The findings are discussed in relation to homeostatic proliferation and their significance in the possible induction of autoimmune disease.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia. Phone: 61 3 8344 5708. Fax: 61 3 9347 1540. E-mail: ccheers{at}unimelb.edu.au.

Editor: J. D. Clements

{dagger} Present address: The Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria 3001, Australia.

Infection and Immunity, December 2004, p. 6884-6891, Vol. 72, No. 12
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.12.6884-6891.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Touvrey, C., Derre, L., Devevre, E., Corthesy, P., Romero, P., Rufer, N., Speiser, D. E. (2009). Dominant Human CD8 T Cell Clonotypes Persist Simultaneously as Memory and Effector Cells in Memory Phase. J. Immunol. 182: 6718-6726 [Abstract] [Full Text]  
  • Nickdel, M B, Conigliaro, P, Valesini, G, Hutchison, S, Benson, R, Bundick, R V, Leishman, A J, McInnes, I B, Brewer, J M, Garside, P (2009). Dissecting the contribution of innate and antigen-specific pathways to the breach of self-tolerance observed in a murine model of arthritis. Ann Rheum Dis 68: 1059-1066 [Abstract] [Full Text]  
  • Mathurin, K. S., Martens, G. W., Kornfeld, H., Welsh, R. M. (2009). CD4 T-Cell-Mediated Heterologous Immunity between Mycobacteria and Poxviruses. J. Virol. 83: 3528-3539 [Abstract] [Full Text]  
  • Bangs, S. C., Baban, D., Cattan, H. J., Li, C. K.-F., McMichael, A. J., Xu, X.-N. (2009). Human CD4+ Memory T Cells Are Preferential Targets for Bystander Activation and Apoptosis. J. Immunol. 182: 1962-1971 [Abstract] [Full Text]  
  • Ramsey, C., Rubinstein, M. P., Kim, D. M., Cho, J.-H., Sprent, J., Surh, C. D. (2008). The Lymphopenic Environment of CD132 (Common {gamma}-Chain)-Deficient Hosts Elicits Rapid Homeostatic Proliferation of Naive T Cells via IL-15. J. Immunol. 180: 5320-5326 [Abstract] [Full Text]  
  • Cho, J.-H., Boyman, O., Kim, H.-O., Hahm, B., Rubinstein, M. P., Ramsey, C., Kim, D. M., Surh, C. D., Sprent, J. (2007). An intense form of homeostatic proliferation of naive CD8+ cells driven by IL-2. JEM 204: 1787-1801 [Abstract] [Full Text]  
  • Kamath, A., Woodworth, J. S.M., Behar, S. M. (2006). Antigen-Specific CD8+ T Cells and the Development of Central Memory during Mycobacterium tuberculosis Infection. J. Immunol. 177: 6361-6369 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»