This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ferraz, J. C.
Right arrow Articles by Tascon, R. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ferraz, J. C.
Right arrow Articles by Tascon, R. E.

 Previous Article  |  Next Article 

Infection and Immunity, December 2004, p. 6945-6950, Vol. 72, No. 12
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.12.6945-6950.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

A Heterologous DNA Priming-Mycobacterium bovis BCG Boosting Immunization Strategy Using Mycobacterial Hsp70, Hsp65, and Apa Antigens Improves Protection against Tuberculosis in Mice

Jose C. Ferraz,1 Evangelos Stavropoulos,1 Min Yang,1 Steve Coade,1 Clara Espitia,2 Douglas B. Lowrie,1 M. Joseph Colston,1 and Ricardo E. Tascon1*

The National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom,1 Departamento de Immunología, Instituto de Investigaciones Biomédicas, UNAM, México-DF, Mexico2

Received 30 April 2004/ Returned for modification 12 June 2004/ Accepted 24 August 2004

Tuberculosis is responsible for >2 million deaths a year, and the number of new cases is rising worldwide. DNA vaccination combined with Mycobacterium bovis bacillus Calmette Guérin (BCG) represents a potential strategy for prevention of this disease. Here, we used a heterologous prime-boost immunization approach using a combination of DNA plasmids and BCG in order to improve the efficacy of vaccination against Mycobacterium tuberculosis infection in mice. As model antigens, we selected the M. tuberculosis Apa (for alanine-proline-rich antigen) and the immunodominant Hsp65 and Hsp70 mycobacterial antigens combined with BCG. We demonstrated that animals injected with a combination of DNA vectors expressing these antigens, when boosted with BCG, showed increased specific antimycobacterial immune responses compared to animals vaccinated with BCG alone. More importantly, the protection achieved with this regimen was also significantly better than with BCG alone.

* Corresponding author. Mailing address: The National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom. Phone: 44-20-89593666. Fax: 44-20-89064477. E-mail: tricard{at}nimr.mrc.ac.uk.

Editor: F. C. Fang

Infection and Immunity, December 2004, p. 6945-6950, Vol. 72, No. 12
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.12.6945-6950.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lin, M. Y., Geluk, A., Smith, S. G., Stewart, A. L., Friggen, A. H., Franken, K. L. M. C., Verduyn, M. J. C., van Meijgaarden, K. E., Voskuil, M. I., Dockrell, H. M., Huygen, K., Ottenhoff, T. H. M., Klein, M. R. (2007). Lack of Immune Responses to Mycobacterium tuberculosis DosR Regulon Proteins following Mycobacterium bovis BCG Vaccination. Infect. Immun. 75: 3523-3530 [Abstract] [Full Text]  
  • Sopp, P., Howard, C. J., Hope, J. C. (2006). Flow Cytometric Detection of Gamma Interferon Can Effectively Discriminate Mycobacterium bovis BCG-Vaccinated Cattle from M. bovis-Infected Cattle. CVI 13: 1343-1348 [Abstract] [Full Text]  
  • Chen, Y.-S., Hsiao, Y.-S., Lin, H.-H., Liu, Y., Chen, Y.-L. (2006). CpG-Modified Plasmid DNA Encoding Flagellin Improves Immunogenicity and Provides Protection against Burkholderia pseudomallei Infection in BALB/c Mice. Infect. Immun. 74: 1699-1705 [Abstract] [Full Text]  
  • Skinner, M. A., Wedlock, D. N., de Lisle, G. W., Cooke, M. M., Tascon, R. E., Ferraz, J. C., Lowrie, D. B., Vordermeier, H. M., Hewinson, R. G., Buddle, B. M. (2005). The Order of Prime-Boost Vaccination of Neonatal Calves with Mycobacterium bovis BCG and a DNA Vaccine Encoding Mycobacterial Proteins Hsp65, Hsp70, and Apa Is Not Critical for Enhancing Protection against Bovine Tuberculosis. Infect. Immun. 73: 4441-4444 [Abstract] [Full Text]  
  • Wu, L., Kong, W.-p., Nabel, G. J. (2005). Enhanced Breadth of CD4 T-Cell Immunity by DNA Prime and Adenovirus Boost Immunization to Human Immunodeficiency Virus Env and Gag Immunogens. J. Virol. 79: 8024-8031 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»