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Infection and Immunity, February 2004, p. 709-716, Vol. 72, No. 2
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.2.709-716.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Binding and Agglutination of Streptococcus pneumoniae by Human Surfactant Protein D (SP-D) Vary between Strains, but SP-D Fails To Enhance Killing by Neutrophils

Rania Jounblat,1 Aras Kadioglu,1* Francesco Iannelli,2 Gianni Pozzi,2 Paul Eggleton,3 and Peter W. Andrew1

Department of Infection, Immunity, and Inflammation, University of Leicester, Leicester, LE1 9HN,1 Institute of Biomedical and Clinical Sciences, Peninsula Medical School, Exeter, EX1 2LU, and MRC Immunochemistry Unit, University of Oxford, Oxford, OX1 3QU, United Kingdom,3 Laboratory of Molecular Microbiology and Biotechnology, Department of Molecular Biology, University of Siena, Siena, Italy2

Received 19 May 2003/ Returned for modification 31 July 2003/ Accepted 23 September 2003

Recombinant human surfactant protein D (SP-D) expressed in Escherichia coli, consisting of the head and neck regions of the native molecule, bound to all strains of Streptococcus pneumoniae that were tested, but the extent of binding varied between strains of differing capsular serotypes. The recombinant protein expressed in the yeast Pichia pastoris did not bind. Full-length native SP-D aggregated pneumococci in a calcium-dependent manner that was inhibited by maltose acting as a competitive sugar. The ability of SP-D to modulate the uptake and killing of pneumococci by human neutrophils was also addressed. Neither recombinant truncated SP-D nor native full-length SP-D enhanced the killing of pneumococci by human neutrophils. Aggregation of pneumococci varied not only between strains of the same multilocus sequence type and different serotypes but also between strains of the same serotype. However, use of recombinant strains in which the serotype had been changed showed that the degree of aggregation was influenced by the capsular type. Indeed, a 19F serotype strain which was not aggregated by SP-D did exhibit aggregation when the original isogenic strain was capsule switched to capsular serotype 3. However, although our results show that SP-D is capable of aggregating most pneumococci, no correlation between the degree of aggregation and the capsule or multilocus sequence type of the pneumococcus was clearly apparent. Therefore, although the capsule serotype is not the only determinant of aggregation by SP-D, the data presented here indicate that it does have a role to play.

* Corresponding author. Mailing address: Department of Infection, Immunity, and Inflammation, P.O. Box 138, University Rd., Leicester, LE1 9HN, United Kingdom. Phone: 44 116 2523018. Fax: 44 116 2525030. E-mail: ak13{at}le.ac.uk.

Editor: A. D. O'Brien

Infection and Immunity, February 2004, p. 709-716, Vol. 72, No. 2
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.2.709-716.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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  • Khamri, W., Moran, A. P., Worku, M. L., Karim, Q. N., Walker, M. M., Annuk, H., Ferris, J. A., Appelmelk, B. J., Eggleton, P., Reid, K. B. M., Thursz, M. R. (2005). Variations in Helicobacter pylori Lipopolysaccharide To Evade the Innate Immune Component Surfactant Protein D. Infect. Immun. 73: 7677-7686 [Abstract] [Full Text]  


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