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Infection and Immunity, March 2004, p. 1306-1310, Vol. 72, No. 3
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.3.1306-1310.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Cryptosporidium parvum-Specific CD4 Th1 Cells from Sensitized Donors Responding to Both Fractionated and Recombinant Antigenic Proteins

Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, 00161 Rome,¹ Department of Experimental Pathology, University of Pisa, 56126 Pisa, Italy²

Received 15 September 2003/ Returned for modification 24 November 2003/ Accepted 2 December 2003

T-cell-mediated immunity plays a central role in the host response to Cryptosporidium parvum. Human T-cell clones (TCC) were isolated from peripheral blood mononuclear cells of five healthy donors with prior cryptosporidiosis by use of a C. parvum crude extract, two antigen fractions obtained by ion-exchange chromatography (IEC1 and IEC2), and two recombinant peptides (SA35 and SA40) from C. parvum sporozoites. The T-cell lines derived from the one recently infected donor had a higher proportion (26 to 38%) of T cells exhibiting the / T-cell receptor (/-TCR) than those from donors who had recovered from cryptosporidiosis several years earlier, suggesting that the / T-cell population is involved in the early stage of the infection. The specific TCC had the /ß-TCR, had the phenotype CD45RO⁺ CD4⁺ CD8^-, and were characterized by either hyperproduction of gamma interferon (IFN-) alone, with a Th1 profile, or IFN- hyperproduction together with interleukin-4 (IL-4) or IL-5 production, with a Th0 profile. SA35, SA40, IEC1, and IEC2 may be considered good targets of the cellular response against C. parvum and may play a role in maintaining the T-cell-mediated memory response to this parasite. Furthermore, the SA35 and SA40 peptides may be regarded as immunodominant antigens involved in the maintenance of the T-cell response in healthy C. parvum-sensitized persons.

Editor: B. B. Finlay

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