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Infection and Immunity, March 2004, p. 1306-1310, Vol. 72, No. 3
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.3.1306-1310.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Cryptosporidium parvum-Specific CD4 Th1 Cells from Sensitized Donors Responding to Both Fractionated and Recombinant Antigenic Proteins

Maria Angeles Gomez Morales,1 Raffaella Mele,1 Alessandra Ludovisi,1 Fabrizio Bruschi,2 Fabio Tosini,1 and Edoardo Pozio1*

Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, 00161 Rome,1 Department of Experimental Pathology, University of Pisa, 56126 Pisa, Italy2

Received 15 September 2003/ Returned for modification 24 November 2003/ Accepted 2 December 2003

T-cell-mediated immunity plays a central role in the host response to Cryptosporidium parvum. Human T-cell clones (TCC) were isolated from peripheral blood mononuclear cells of five healthy donors with prior cryptosporidiosis by use of a C. parvum crude extract, two antigen fractions obtained by ion-exchange chromatography (IEC1 and IEC2), and two recombinant peptides (SA35 and SA40) from C. parvum sporozoites. The T-cell lines derived from the one recently infected donor had a higher proportion (26 to 38%) of T cells exhibiting the {gamma}/{delta} T-cell receptor ({gamma}/{delta}-TCR) than those from donors who had recovered from cryptosporidiosis several years earlier, suggesting that the {gamma}/{delta} T-cell population is involved in the early stage of the infection. The specific TCC had the {alpha}/ß-TCR, had the phenotype CD45RO+ CD4+ CD8-, and were characterized by either hyperproduction of gamma interferon (IFN-{gamma}) alone, with a Th1 profile, or IFN-{gamma} hyperproduction together with interleukin-4 (IL-4) or IL-5 production, with a Th0 profile. SA35, SA40, IEC1, and IEC2 may be considered good targets of the cellular response against C. parvum and may play a role in maintaining the T-cell-mediated memory response to this parasite. Furthermore, the SA35 and SA40 peptides may be regarded as immunodominant antigens involved in the maintenance of the T-cell response in healthy C. parvum-sensitized persons.

* Corresponding author. Mailing address: Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Phone: 3906 4990 2304. Fax: 3906 4938 7065. E-mail: pozio{at}iss.it.

Editor: B. B. Finlay

Infection and Immunity, March 2004, p. 1306-1310, Vol. 72, No. 3
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.3.1306-1310.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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