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Infection and Immunity, March 2004, p. 1804-1806, Vol. 72, No. 3
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.3.1804-1806.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Rat Neutrophils Prevent the Development of Tuberculosis

Isamu Sugawara,* Tadashi Udagawa, and Hiroyuki Yamada

Mycobacterial Reference Center, The Research Institute of Tuberculosis, Tokyo 204-0022, Japan

Received 31 July 2003/ Returned for modification 14 September 2003/ Accepted 9 October 2003

To understand the role of neutrophils in the development of rat tuberculosis in vivo, we utilized lipopolysaccharide (LPS)-induced neutrophilia in the lungs. LPS (50 µg/ml) was administered intratracheally to male Fischer rats. Rats were then infected with Mycobacterium tuberculosis by an airborne route. Intratracheal injection of LPS significantly blocked the development of pulmonary granulomas and significantly reduced pulmonary CFU (P < 0.01). LPS treatment with amphotericin B (an LPS inhibitor) or neutralizing anti-rat neutrophil antibody reversed the development of pulmonary lesions. LPS-induced transient neutrophilia prevented early mycobacterial infection. The timing of LPS administration was important. When given intratracheally at least 10 days after aerial infection, LPS did not prevent development of tuberculosis. Neutrophils obtained by bronchoalveolar lavage killed M. tuberculosis cells. These results indicate clearly that neutrophils participate actively in defense against early-phase tuberculosis.

* Corresponding author. Mailing address: Mycobacterial Reference Center, The Research Institute of Tuberculosis, 3-1-24 Matsuyama, Kiyose, Tokyo 204-0022, Japan. Phone: 81 424 93 5075. Fax: 81 424 92 4600. E-mail: sugawara{at}jata.or.jp.

Editor: J. T. Barbieri

Infection and Immunity, March 2004, p. 1804-1806, Vol. 72, No. 3
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.3.1804-1806.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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