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Infection and Immunity, April 2004, p. 1906-1913, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.1906-1913.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Moraxella catarrhalis Porin-Like Outer Membrane Protein CD Is an Adhesin for Human Lung Cells

Melissa M. Holm, Serena L. Vanlerberg, Ian M. Foley, Darren D. Sledjeski, and Eric R. Lafontaine*

Department of Microbiology and Immunology, Medical College of Ohio, Toledo, Ohio 43614-5806

Received 4 August 2003/ Returned for modification 4 November 2003/ Accepted 27 December 2003

The outer membrane protein CD (OMPCD) of Moraxella catarrhalis is an outer membrane protein with several attributes of a potential vaccine antigen. We isolated four transposon mutants of strain O35E on the basis of their reduced binding to A549 human lung cells in microcolony formation assays, and we determined that they contain a transposon in ompCD. We also found that these transposon insertions had pleiotropic effects: mutants grew slower, became serum sensitive, bound ~10-fold less to A549 cells, and appeared transparent when grown on solid medium. We confirmed that these various phenotypes could be attributed solely to disruption of ompCD by constructing the isogenic strain O35E.CD1. O35E-ompCD was cloned, and recombinant Escherichia coli bacteria expressing the gene product exhibited a 10-fold increase in adherence to A549 cells. This is the first report of M. catarrhalis ompCD mutants, and our findings demonstrate that this gene product is an adhesin for human lung cells.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Medical College of Ohio, Health Education Building, 3055 Arlington Ave., Toledo, Ohio 43614-5806. Phone: (419) 383-6626. Fax: (419) 383-3002. E-mail: elafontaine{at}mco.edu.

Editor: V. J. DiRita

Infection and Immunity, April 2004, p. 1906-1913, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.1906-1913.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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