This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ASM journals
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miki, K.
Right arrow Articles by Koide, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miki, K.
Right arrow Articles by Koide, Y.

 Previous Article  |  Next Article 

Infection and Immunity, April 2004, p. 2014-2021, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.2014-2021.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51

Keita Miki,1,2 Toshi Nagata,1* Takao Tanaka,3 Yeung-Hyen Kim,1 Masato Uchijima,1 Naoya Ohara,4 Satoshi Nakamura,2 Masaji Okada,3 and Yukio Koide1

Department of Microbiology and Immunology,1 Second Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu,2 Clinical Research Center, National Kinki-Chuo Hospital for Chest Diseases, Osaka,3 Department of Developmental and Reconstructive Medicine, Division of Microbiology and Oral Infection, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan4

Received 29 October 2003/ Returned for modification 16 December 2003/ Accepted 7 January 2004

We report here the induction of specific protective cellular immunity against Mycobacterium tuberculosis by the employment of vaccination with recombinant attenuated Listeria monocytogenes strains. We constructed self-destructing attenuated L. monocytogenes {Delta}2 strains carrying eukaryotic expression plasmids for the antigen 85 complex (Ag85A and Ag85B) and for MPB/MPT51 (mycobacterial protein secreted by M. bovis BCG/mycobacterial protein secreted by M. tuberculosis) molecules. Infection of these recombinant bacteria allowed expression of the genes in the J774A.1 murine macrophage cell line. Intraperitoneal vaccination of C57BL/6 mice with these recombinant bacteria was capable of inducing purified protein derivative-specific cellular immune responses, such as foot pad reactions, proliferative responses of splenocytes, and gamma interferon production from splenocytes, suggesting the efficacy of vaccination against mycobacterial infection by use of these recombinant L. monocytogenes strains. Furthermore, intravenous vaccination with recombinant bacteria carrying expression plasmids for Ag85A, Ag85B, or MPB/MPT51 in BALB/c mice elicited significant protective responses, comparable to those evoked by a live Mycobacterium bovis BCG vaccine. Notably, this is the first report to show that MPB/MPT51 is a major protective antigen in addition to Ag85A and Ag85B, which have been reported to be major mycobacterial protective antigens.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu 431-3192, Japan. Phone and fax: 81-53-435-2335. E-mail: tnagata{at}hama-med.ac.jp.

Editor: S. H. E. Kaufmann

Infection and Immunity, April 2004, p. 2014-2021, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.2014-2021.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Aoshi, T., Nagata, T., Suzuki, M., Uchijima, M., Hashimoto, D., Rafiei, A., Suda, T., Chida, K., Koide, Y. (2008). Identification of an HLA-A*0201-Restricted T-Cell Epitope on the MPT51 Protein, a Major Secreted Protein Derived from Mycobacterium tuberculosis, by MPT51 Overlapping Peptide Screening. Infect. Immun. 76: 1565-1571 [Abstract] [Full Text]  
  • Li, Z., Zhao, X., Zhou, C., Gu, B., Frankel, F. R. (2006). A truncated Bacillus subtilis dal gene with a 3' ssrA gene tag regulates the growth and virulence of racemase-deficient Listeria monocytogenes.. Microbiology 152: 3091-3102 [Abstract] [Full Text]  
  • Zhao, X., Zhang, M., Li, Z., Frankel, F. R. (2006). Vaginal Protection and Immunity after Oral Immunization of Mice with a Novel Vaccine Strain of Listeria monocytogenes Expressing Human Immunodeficiency Virus Type 1 gag. J. Virol. 80: 8880-8890 [Abstract] [Full Text]  
  • Loeffler, D. I. M., Schoen, C. U., Goebel, W., Pilgrim, S. (2006). Comparison of Different Live Vaccine Strategies In Vivo for Delivery of Protein Antigen or Antigen-Encoding DNA and mRNA by Virulence-Attenuated Listeria monocytogenes. Infect. Immun. 74: 3946-3957 [Abstract] [Full Text]  
  • Torres, M., Ramachandra, L., Rojas, R. E., Bobadilla, K., Thomas, J., Canaday, D. H., Harding, C. V., Boom, W. H. (2006). Role of Phagosomes and Major Histocompatibility Complex Class II (MHC-II) Compartment in MHC-II Antigen Processing of Mycobacterium tuberculosis in Human Macrophages. Infect. Immun. 74: 1621-1630 [Abstract] [Full Text]  
  • Ko, H.-J., Ko, S.-Y., Kim, Y.-J., Lee, E.-G., Cho, S.-N., Kang, C.-Y. (2005). Optimization of Codon Usage Enhances the Immunogenicity of a DNA Vaccine Encoding Mycobacterial Antigen Ag85B. Infect. Immun. 73: 5666-5674 [Abstract] [Full Text]  
  • Zhao, X., Li, Z., Gu, B., Frankel, F. R. (2005). Pathogenicity and Immunogenicity of a Vaccine Strain of Listeria monocytogenes That Relies on a Suicide Plasmid To Supply an Essential Gene Product. Infect. Immun. 73: 5789-5798 [Abstract] [Full Text]  
  • Kashyap, R. S., Dobos, K. M., Belisle, J. T., Purohit, H. J., Chandak, N. H., Taori, G. M., Daginawala, H. F. (2005). Demonstration of Components of Antigen 85 Complex in Cerebrospinal Fluid of Tuberculous Meningitis Patients. CVI 12: 752-758 [Abstract] [Full Text]  
  • Hovav, A.-H., Fishman, Y., Bercovier, H. (2005). Gamma Interferon and Monophosphoryl Lipid A-Trehalose Dicorynomycolate Are Efficient Adjuvants for Mycobacterium tuberculosis Multivalent Acellular Vaccine. Infect. Immun. 73: 250-257 [Abstract] [Full Text]  
  • Ronning, D. R., Vissa, V., Besra, G. S., Belisle, J. T., Sacchettini, J. C. (2004). Mycobacterium tuberculosis Antigen 85A and 85C Structures Confirm Binding Orientation and Conserved Substrate Specificity. J. Biol. Chem. 279: 36771-36777 [Abstract] [Full Text]  
  • Suzuki, M., Aoshi, T., Nagata, T., Koide, Y. (2004). Identification of Murine H2-Dd- and H2-Ab-Restricted T-Cell Epitopes on a Novel Protective Antigen, MPT51, of Mycobacterium tuberculosis. Infect. Immun. 72: 3829-3837 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»