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Infection and Immunity, April 2004, p. 2022-2028, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.2022-2028.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Nature of Extracellular Iron Influences Iron Acquisition by Mycobacterium tuberculosis Residing within Human Macrophages

Oyebode Olakanmi,1,2 Larry S. Schlesinger,3 Ambar Ahmed,1 and Bradley E. Britigan1,2,4*

Department of Internal Medicine and Research Service, VA Medical Center—Iowa City, Iowa City, Iowa 52246,1 Departments of Internal Medicine,2 The Free Radical and Radiation Biology Program, Roy G. and Lucille A. Carver College of Medicine, The University of Iowa, Iowa City, Iowa 52242,4 Departments of Internal Medicine and Molecular Virology, Immunology, and Medical Genetics and The Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio 432103

Received 29 October 2003/ Returned for modification 5 December 2003/ Accepted 16 December 2003

We have reported that Mycobacterium tuberculosis residing within the phagosomes of human monocyte-derived macrophages (MDM) can acquire Fe from extracellular transferrin (TF) and sources within the MDM. In the lung, Fe is also bound to lactoferrin (LF) and low-molecular-weight chelates. We therefore investigated the ability of intraphagosomal M. tuberculosis to acquire Fe from these sources. M. tuberculosis acquired 30-fold and 3-fold more Fe from LF and citrate, respectively, compared to TF, in spite of similar MDM-associated Fe. M. tuberculosis infection decreased MDM-associated Fe relative to uninfected MDM as follows: TF (38.7%), citrate (21.1%), and LF (15.3%). M. tuberculosis Fe acquisition from extracellular chelates (exogenous source) and from endogenous MDM Fe initially acquired from the three chelates (endogenous source) was compared. M. tuberculosis Fe acquisition was similar from exogenous and endogenous sources supplied as Fe-TF. In contrast, there was much greater intracellular M. tuberculosis Fe uptake from LF and citrate from the exogenous than endogenous source. Gamma interferon (IFN-{gamma}) reduced MDM Fe uptake from each chelate by ~50% and augmented the M. tuberculosis-induced decrease in MDM Fe uptake from exogenous TF, but not from LF or citrate. IFN-{gamma} minimally decreased intracellular M. tuberculosis Fe acquisition from exogenous Fe-TF but significantly increased Fe uptake from LF and citrate. Intraphagosomal M. tuberculosis Fe acquisition from both exogenous and endogenous MDM sources, and the effect of IFN-{gamma} on this process, is influenced by the nature of the extracellular Fe chelate. M. tuberculosis has developed efficient mechanisms of acquiring Fe from a variety of Fe chelates that it likely encounters within the human lung.

* Correpsonding author. Mailing address: University of Iowa Hospitals and Clinics, Department of Internal Medicine, SW54, GH, Iowa City, IA 52242. Phone: (319) 356-3674. Fax: (319) 356-4600. E-mail: bradley-britigan{at}uiowa.edu.

Editor: F. C. Fang

Infection and Immunity, April 2004, p. 2022-2028, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.2022-2028.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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