This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Buza, J. J.
Right arrow Articles by Momotani, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Buza, J. J.
Right arrow Articles by Momotani, E.

 Previous Article  |  Next Article 

Infection and Immunity, April 2004, p. 2425-2428, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.2425-2428.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Neutralization of Interleukin-10 Significantly Enhances Gamma Interferon Expression in Peripheral Blood by Stimulation with Johnin Purified Protein Derivative and by Infection with Mycobacterium avium subsp. paratuberculosis in Experimentally Infected Cattle with Paratuberculosis

Joram J. Buza,1 Hirokazu Hikono,1 Yasuyuki Mori,2 Reiko Nagata,2 Sachiyo Hirayama,1 Abusaleh M. Bari,1 Aodon-geril,,1 Yujing Shu,1 Noriko M. Tsuji,3 and Eiichi Momotani1*

Paratuberculosis and Inflammatory Bowel Disease Research Team,1 Immune System Section, National Institute of Animal Health,2 Molecular Immunology Laboratory, National Institute of Agrobiological Sciences, 3-1-5 Kan-nondai, Tsukuba 305-0856, Japan3

Received 28 October 2003/ Returned for modification 16 December 2003/ Accepted 8 January 2004

Monoclonal antibody neutralization of interleukin-10 (IL-10) increased Johnin purified protein derivative-induced whole-blood gamma interferon (IFN-{gamma}) secretion 23-fold and also increased IFN-{gamma} secretion ninefold following in vitro Mycobacterium avium subsp. paratuberculosis infection of peripheral blood mononuclear cells. These results demonstrate the suppressive effect of IL-10 on immune responses to M. avium subsp. paratuberculosis infection in cattle.

* Corresponding author. Mailing address: Paratuberculosis and Inflammatory Bowel Disease Research Team, NIAH, 3-1-5 Kan-nondai, Tsukuba 305-0856, Japan. Phone: 81-29-838-7781. Fax: 81-29-838-7781. E-mail: momotani{at}affrc.go.jp.

Editor: S. H. E. Kaufmann

Infection and Immunity, April 2004, p. 2425-2428, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.2425-2428.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lybeck, K. R., Storset, A. K., Olsen, I. (2009). Neutralization of Interleukin-10 from CD14+ Monocytes Enhances Gamma Interferon Production in Peripheral Blood Mononuclear Cells from Mycobacterium avium subsp. paratuberculosis-Infected Goats. CVI 16: 1003-1011 [Abstract] [Full Text]  
  • Begg, D. J., de Silva, K., Bosward, K., Di Fiore, L., Taylor, D. L., Jungersen, G., Whittington, R. J. (2009). Enzyme-linked immunospot: an alternative method for the detection of interferon gamma in Johne's disease. jvdi 21: 187-196 [Abstract] [Full Text]  
  • Weiss, D. J., Souza, C. D. (2008). REVIEW PAPER: Modulation of Mononuclear Phagocyte Function by Mycobacterium avium subsp. paratuberculosis. Vet Pathol 45: 829-841 [Abstract] [Full Text]  
  • Denis, M., Wedlock, D. N., McCarthy, A. R., Parlane, N. A., Cockle, P. J., Vordermeier, H. M., Hewinson, R. G., Buddle, B. M. (2007). Enhancement of the Sensitivity of the Whole-Blood Gamma Interferon Assay for Diagnosis of Mycobacterium bovis Infections in Cattle. CVI 14: 1483-1489 [Abstract] [Full Text]  
  • Weiss, D. J., Evanson, O. A., Souza, C. D. (2006). Mucosal Immune Response in Cattle with Subclinical Johne's Disease.. Vet Pathol 43: 127-135 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»