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Infection and Immunity, May 2004, p. 2858-2863, Vol. 72, No. 5
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.5.2858-2863.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Cleavage of Complement C3b to iC3b on the Surface of Staphylococcus aureus Is Mediated by Serum Complement Factor I

K. M. Cunnion,* P. S. Hair, and E. S. Buescher

Center for Pediatric Research, Eastern Virginia Medical School and Children's Hospital for The King's Daughters, Norfolk, Virginia

Received 5 December 2003/ Returned for modification 8 January 2004/ Accepted 27 January 2004

Complement-mediated opsonization of Staphylococcus aureus bearing the dominant capsule serotypes, serotypes 5 and 8, remains incompletely understood. We have previously shown that complement plays a vital role in the efficient phagocytosis of a serotype 5 S. aureus strain and that the opsonic fragments of the central complement protein C3, C3b and iC3b, were present on the bacterial surface after incubation in human serum. In the present studies, C3b and iC3b were found on several serotype 5 and 8 S. aureus strains after incubation in human serum. Using purified classical activation pathway complement proteins and the Western blot assay, we showed that when C3b was generated on the S. aureus surface no iC3b fragments were found, suggesting that other serum proteins may be required for cleaving C3b to iC3b. When C3b-coated S. aureus was incubated with serum factor I, a complement regulatory protein, iC3b was generated. Purified factor H, a serum protein cofactor for factor I, did not enhance factor I-mediated cleavage of C3b. These findings suggest that C3b cleavage to iC3b on S. aureus is mediated by serum factor I and does not require factor H.

* Corresponding author. Mailing address: Center for Pediatric Research, 855 W. Brambleton Ave., Norfolk, VA 23510. Phone: (757) 668-7238. Fax: (757) 668-8275. E-mail: cunniok{at}chkd.org.

Editor: T. R. Kozel

Infection and Immunity, May 2004, p. 2858-2863, Vol. 72, No. 5
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.5.2858-2863.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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