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Infection and Immunity, June 2004, p. 3693-3694, Vol. 72, No. 6
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.6.3693-3694.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Growth of Piscirickettsia salmonis to High Titers in Insect Tissue Culture Cells

T. Harry Birkbeck,1* Angela A. Griffen,1 Helen I. Reid,1 L. Anthony Laidler,2 and Simon Wadsworth2,{dagger}

Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of GlasgowGlasgow,1 Marine Harvest Ltd., Lochailort, Inverness-shire, Scotland, United Kingdom2

Received 19 December 2003/ Returned for modification 7 February 2004/ Accepted 12 February 2004

Piscirickettsia salmonis was grown in established insect, frog, and fish tissue culture cells. The yield of P. salmonis in Sf21 cells was up to 100 times that obtained in CHSE-214 cells, and virulence for Atlantic salmon was retained. The ceiling temperature for growth of P. salmonis in Sf21 cells was 24°C.

* Corresponding author. Mailing address: Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, Scotland Gl2 8QQ. Phone: (44)141 3305483. Fax: (44) 141 3304600. E-mail: T.H.Birkbeck{at}bio.gla.ac.uk.

Editor: J. T. Barbieri

{dagger} Present address: EWOS Innovation, Dirdal, N-4335 Norway.

Infection and Immunity, June 2004, p. 3693-3694, Vol. 72, No. 6
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.6.3693-3694.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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