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Infection and Immunity, August 2004, p. 4416-4423, Vol. 72, No. 8
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.8.4416-4423.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Induction of Maturation and Cytokine Release of Human Dendritic Cells by Helicobacter pylori

Katharina Kranzer,1 Alexander Eckhardt,1 Michael Aigner,1 Gertrud Knoll,1 Ludwig Deml,1 Cornelia Speth,2 Norbert Lehn,1 Michael Rehli,3 and Wulf Schneider-Brachert1*

Institute for Medical Microbiology and Hygiene,1 Department of Hematology and Oncology, University of Regensburg, D-93053 Regensburg, Germany,3 Institute of Hygiene and Social Medicine, University of Innsbruck, A-6020 Innsbruck, Austria2

Received 22 December 2003/ Returned for modification 13 February 2004/ Accepted 7 April 2004

Helicobacter pylori causes a persistent infection in the human stomach, which can result in chronic gastritis and peptic ulcer disease. Despite an intensive proinflammatory response, the immune system is not able to clear the organism. However, the immune escape mechanisms of this common bacterium are not well understood. We investigated the interaction between H. pylori and human dendritic cells. Dendritic cells (DCs) are potent antigen-presenting cells and important mediators between the innate and acquired immune system. Stimulation of DCs with different concentrations of H. pylori for 8, 24, 48, and 72 h resulted in dose-dependent interleukin-6 (IL-6), IL-8, IL-10 and IL-12 production. Lipopolysaccharide (LPS) from Escherichia coli, a known DC maturation agent, was used as a positive control. The cytokine release after stimulation with LPS was comparable to that induced by H. pylori except for IL-12. After LPS stimulation IL-12 was only moderately released compared to the large amounts of IL-12 induced by H. pylori. We further investigated the potential of H. pylori to induce maturation of DCs. Fluorescence-activated cell sorting analysis of cell surface expression of maturation marker molecules such as CD80, CD83, CD86, and HLA-DR revealed equal upregulation after stimulation with H. pylori or LPS. We found no significant differences between H. pylori seropositive and seronegative donors of DCs with regard to cytokine release and upregulation of surface molecules. These data clearly demonstrate that H. pylori induces a strong activation and maturation of human immature DCs.

* Corresponding author. Mailing address: Institute for Medical Microbiology and Hygiene, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany. Phone: 49-941-944-6461. Fax: 49-941-944-6402. E-mail: wulf.schneider{at}klinik.uni-regensburg.de.

Editor: J. B. Bliska

Infection and Immunity, August 2004, p. 4416-4423, Vol. 72, No. 8
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.8.4416-4423.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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