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Infection and Immunity, August 2004, p. 4848-4858, Vol. 72, No. 8
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.8.4848-4858.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Aeromonas hydrophila Beta-Hemolysin Induces Active Chloride Secretion in Colon Epithelial Cells (HT-29/B6)

H. J. Epple,1 J. Mankertz,1 R. Ignatius,2 O. Liesenfeld,2 M. Fromm,3 M. Zeitz,1 T. Chakraborty,4 and J. D. Schulzke1*

Medical Clinic I, Gastroenterology, Infectiology, and Rheumatology,1 Institute of Clinical Physiology,2 Institute of Medicine of Infection, Charité-University Medicine, Berlin,3 Department of Medical Microbiology and Virology, Justus-Liebig-University, Giessen, Germany4

Received 18 November 2003/ Returned for modification 5 February 2004/ Accepted 2 April 2004

The diarrheal mechanisms in Aeromonas enteritis are not completely understood. In this study we investigated the effect of aeromonads and of their secretory products on ion secretion and barrier function of monolayers of human intestinal cells (HT-29/B6). Ion secretion was determined as a short-circuit current (ISC) of HT-29/B6 monolayers mounted in Ussing-type chambers. Transepithelial resistance (Rt) served as a measure of permeability. A diarrheal strain of Aeromonas hydrophila (strain Sb) added to the mucosal side of HT-29/B6 monolayers induced a significant ISC (39 ± 3 µA/cm2) and decreased the Rt to ~10% of the initial value. A qualitatively identical response was obtained with sterile supernatant of strain Sb, and Aeromonas supernatant also induced a significant ISC in totally stripped human colon. Tracer flux and ion replacement studies revealed the ISC to be mainly accounted for by electrogenic Cl– secretion. Supernatant applied serosally completely abolished basal ISC. The supernatant-induced ISC was inhibited by the protein kinase C inhibitor chelerythrine, whereas a protein kinase A inhibitor (H8) and a Ca2+ chelator (BAPTA-AM) had no effect. Physicochemical properties indicated that the supernatant's active compound was an aerolysin-related Aeromonas beta-hemolysin. Accordingly, identical ISC and Rt responses were obtained with Escherichia coli lysates harboring the cloned beta-hemolysin gene from strain SB or the aerA gene encoding for aerolysin. Sequence comparison revealed a 64% homology between aerolysin and the beta-hemolysin cloned from Aeromonas sp. strain Sb. In conclusion, beta-hemolysin secreted by pathogenic aeromonads induces active Cl– secretion in the intestinal epithelium, possibly by channel insertion into the apical membrane and by activation of protein kinase C.

* Corresponding author. Mailing address: Medizinische Klinik I, Gastroenterologie, Infektiologie, und Rheumatologie, Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany. Phone: 49(0)30-8445-2666. Fax: 49(0)30-8445-4493. E-mail: joerg.schulzke{at}charite.de.

Editor: F. C. Fang

Infection and Immunity, August 2004, p. 4848-4858, Vol. 72, No. 8
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.8.4848-4858.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Galindo, C. L., Fadl, A. A., Sha, J., Pillai, L., Gutierrez, C. Jr., Chopra, A. K. (2005). Microarray and Proteomics Analyses of Human Intestinal Epithelial Cells Treated with the Aeromonas hydrophila Cytotoxic Enterotoxin. Infect. Immun. 73: 2628-2643 [Abstract] [Full Text]  


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