This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by John, C. C.
Right arrow Articles by Kazura, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by John, C. C.
Right arrow Articles by Kazura, J. W.

 Previous Article  |  Next Article 

Infection and Immunity, September 2004, p. 5135-5142, Vol. 72, No. 9
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.9.5135-5142.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Gamma Interferon Responses to Plasmodium falciparum Liver-Stage Antigen 1 and Thrombospondin-Related Adhesive Protein and Their Relationship to Age, Transmission Intensity, and Protection against Malaria

Chandy C. John,1,2,{dagger}* Ann M. Moormann,1,{dagger} Peter O. Sumba,3 Ayub V. Ofulla,4 Daniel C. Pregibon,1 and James W. Kazura1

Center for Global Health and Diseases,1 Division of Pediatric Infectious Diseases, Case Western Reserve University, and Rainbow Center for International Child Health, Rainbow Babies and Children's Hospital, Cleveland, Ohio,2 Center for Vector Biology and Control Research, Kenya Medical Research Institute, Kisian,3 Department of Biomedical Sciences and Technology, Maseno University, Maseno, Kenya4

Received 1 April 2004/ Returned for modification 22 May 2004/ Accepted 1 June 2004

Gamma interferon (IFN-{gamma}) responses to the Plasmodium falciparum antigens liver-stage antigen 1 (LSA-1) and thrombospondin-related adhesive protein (TRAP) are thought to be important in protection against malaria. Optimal methods of testing and the effects of age and transmission intensity on these responses are unknown. IFN-{gamma} responses to LSA-1 and TRAP peptides were assessed by the enzyme-linked immunospot assay (ELISPOT) and enzyme-linked immunosorbent assay (ELISA) in children and adults from areas of stable and unstable malaria transmission in Kenya. Adults in the areas of stable and unstable transmission had similar frequencies and levels of IFN-{gamma} responses to LSA-1 and TRAP as determined by ELISPOT and ELISA. In contrast, IFN-{gamma} responses to the LSA-1 T3 peptide (assessed by ELISPOT) and to any LSA-1 peptide (assessed by ELISA) were less frequent in children in the area of unstable transmission than in children in the area of stable transmission. IFN-{gamma} responses to LSA-1 were more frequently detected by ELISA than by ELISPOT in the stable-transmission area. IFN-{gamma} responses detected by ELISA and ELISPOT did not correlate with each other. In children in the stable-transmission area, IFN-{gamma} responses to LSA-1 peptides assessed by ELISA, but not by ELISPOT, were associated with protection against clinical malaria and anemia. IFN-{gamma} responses to LSA-1 appear to require repeated P. falciparum exposure and/or increased age and, as measured by ELISA, are associated with protection against clinical malaria and anemia.

* Corresponding author. Mailing address: Rainbow Center for International Child Health, Rainbow Babies and Children's Hospital, RBC 487, 11100 Euclid Ave., MS6008, Cleveland, OH 44106. Phone: (216) 844-3645. Fax: (216) 368-4825. E-mail: chandy.john{at}case.edu.

Editor: W. A. Petri, Jr.

{dagger} C.C.J. and A.M.M. contributed equally to this work.

Infection and Immunity, September 2004, p. 5135-5142, Vol. 72, No. 9
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.9.5135-5142.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Moormann, A. M., Sumba, P. O., Tisch, D. J., Embury, P., King, C. H., Kazura, J. W., John, C. C. (2009). Stability of Interferon-Gamma and Interleukin-10 Responses to Plasmodium falciparum Liver Stage Antigen 1 and Thrombospondin-Related Adhesive Protein Immunodominant Epitopes in a Highland Population from Western Kenya. Am J Trop Med Hyg 81: 489-495 [Abstract] [Full Text]  
  • Noland, G. S., Hendel-Paterson, B., Min, X. M., Moormann, A. M., Vulule, J. M., Narum, D. L., Lanar, D. E., Kazura, J. W., John, C. C. (2008). Low Prevalence of Antibodies to Preerythrocytic but Not Blood-Stage Plasmodium falciparum Antigens in an Area of Unstable Malaria Transmission Compared to Prevalence in an Area of Stable Malaria Transmission. Infect. Immun. 76: 5721-5728 [Abstract] [Full Text]  
  • Rodriguez, A., Goudsmit, J., Companjen, A., Mintardjo, R., Gillissen, G., Tax, D., Sijtsma, J., Weverling, G. J., Holterman, L., Lanar, D. E., Havenga, M. J. E., Radosevic, K. (2008). Impact of Recombinant Adenovirus Serotype 35 Priming versus Boosting of a Plasmodium falciparum Protein: Characterization of T- and B-Cell Responses to Liver-Stage Antigen 1. Infect. Immun. 76: 1709-1718 [Abstract] [Full Text]  
  • Malhotra, I., Wamachi, A. N., Mungai, P. L., Mzungu, E., Koech, D., Muchiri, E., Moormann, A. M., King, C. L. (2008). Fine Specificity of Neonatal Lymphocytes to an Abundant Malaria Blood-Stage Antigen: Epitope Mapping of Plasmodium falciparum MSP133. J. Immunol. 180: 3383-3390 [Abstract] [Full Text]  
  • MOORMANN, A. M., JOHN, C. C., SUMBA, P. O., TISCH, D., EMBURY, P., KAZURA, J. W. (2006). STABILITY OF INTERFERON-{gamma} AND INTERLEUKIN-10 RESPONSES TO PLASMODIUM FALCIPARUM LIVER STAGE ANTIGEN-1 AND THROMBOSPONDIN-RELATED ADHESIVE PROTEIN IN RESIDENTS OF A MALARIA HOLOENDEMIC AREA. Am J Trop Med Hyg 74: 585-590 [Abstract] [Full Text]  
  • JOHN, C. C., MOORMANN, A. M., PREGIBON, D. C., SUMBA, P. O., MCHUGH, M. M., NARUM, D. L., LANAR, D. E., SCHLUCHTER, M. D., KAZURA, J. W. (2005). CORRELATION OF HIGH LEVELS OF ANTIBODIES TO MULTIPLE PRE-ERYTHROCYTIC PLASMODIUM FALCIPARUM ANTIGENS AND PROTECTION FROM INFECTION. Am J Trop Med Hyg 73: 222-228 [Abstract] [Full Text]  
  • Hillier, C. J., Ware, L. A., Barbosa, A., Angov, E., Lyon, J. A., Heppner, D. G., Lanar, D. E. (2005). Process Development and Analysis of Liver-Stage Antigen 1, a Preerythrocyte-Stage Protein-Based Vaccine for Plasmodium falciparum. Infect. Immun. 73: 2109-2115 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»