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Infection and Immunity, September 2004, p. 5168-5174, Vol. 72, No. 9
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.9.5168-5174.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Chemokine Gene Expression in Toll-Like Receptor-Competent and -Deficient Mice Infected with Leishmania major

Simone Antoniazi,1,2 Helen P. Price,2 Pascale Kropf,1 Marina A. Freudenberg,3 Chris Galanos,3 Deborah F. Smith,2 and Ingrid Müller1*

Department of Immunology, Faculty of Medicine,1 Wellcome Trust Laboratories for Molecular Parasitology, Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Faculty of Life Sciences, Imperial College London, London, United Kingdom,2 Max-Planck-Institut für Immunbiologie, Freiburg, Germany3

Received 23 December 2003/ Returned for modification 17 February 2004/ Accepted 1 June 2004

We studied the expression of a subset of chemokines, including RANTES/CCL5, MIP-1{alpha}/CCL3, IP-10/CXCL10, and MCP-1/CCL2, in Toll-like receptor (TLR)-competent and -deficient mice after infection with Leishmania major. Chemokine expression at the site of infection (the footpad), in the draining lymph nodes and in the spleens of infected animals was determined by using two different methods of analysis. The results indicate that L. major infection causes overall upregulation of RANTES/CCL5, MIP-1{alpha}/CCL3, IP-10/CXCL10, and MCP-1/CCL2 in the footpads and lymph nodes, while expression of these chemokines is constitutive in the spleens of TLR4-competent mice (C57BL/10ScSn) and TLR4-deficient mice (C57BL10/ScN). Different patterns of expression were detected depending on the time postinfection, but there was little variation in the expression of these four chemokines in the presence or absence of TLR4.

* Corresponding author. Mailing address: Imperial College London, Faculty of Medicine, Department of Immunology, Norfolk Place, London W2 1PG, United Kingdom. Phone: 44-020 7594 3732. Fax: 44-20 7402 0653. E-mail: i.muller{at}imperial.ac.uk.

Editor: S. H. E. Kaufmann

Infection and Immunity, September 2004, p. 5168-5174, Vol. 72, No. 9
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.9.5168-5174.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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