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Infection and Immunity, January 2005, p. 609-611, Vol. 73, No. 1
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.1.609-611.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Development of a Chinchilla Model To Allow Direct, Continuous, Biophotonic Imaging of Bioluminescent Nontypeable Haemophilus influenzae during Experimental Otitis Media

Laura A. Novotny, Kevin M. Mason, and Lauren O. Bakaletz*

Department of Pediatrics, Columbus Children’s Research Institute, The Ohio State University College of Medicine and Public Health, Columbus, Ohio

Received 23 June 2004/ Returned for modification 30 July 2004/ Accepted 20 September 2004

We transformed a nontypeable Haemophilus influenzae clinical isolate with a plasmid containing the luxCDABE operon driven by the H. influenzae outer membrane protein P2 promoter. Herein, we demonstrate the ability to detect bioluminescence and to monitor infection within the nasopharynges, eustachian tubes, and middle ears of chinchillas after intranasal and transbullar challenges.

* Corresponding author. Mailing address: Center for Microbial Pathogenesis, Columbus Children's Research Institute, The Ohio State University College of Medicine and Public Health, Rm. W591, 700 Children's Dr., Columbus, OH 43205-2696. Phone: (614) 722-2915. Fax: (614) 722-2818. E-mail: BakaletL{at}pediatrics.ohio-state.edu.

Editor: D. L. Burns

Infection and Immunity, January 2005, p. 609-611, Vol. 73, No. 1
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.1.609-611.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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