This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jost, B. H.
Right arrow Articles by Songer, J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jost, B. H.
Right arrow Articles by Songer, J. G.

 Previous Article  |  Next Article 

Infection and Immunity, January 2005, p. 652-656, Vol. 73, No. 1
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.1.652-656.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Atypical cpb2 Genes, Encoding Beta2-Toxin in Clostridium perfringens Isolates of Nonporcine Origin

B. Helen Jost,* Stephen J. Billington, Hien T. Trinh, Dawn M. Bueschel, and J. Glenn Songer

Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona

Received 25 August 2004/ Accepted 3 September 2004

Beta2-toxin, encoded by cpb2, is implicated in the pathogenesis of Clostridium perfringens enteritis. However, cpb2 genes from nonporcine C. perfringens isolates were not always expressed, at least in vitro. Nucleotide sequencing identified atypical cpb2 genes with 70.2 to 70.7% DNA identity to previously identified (consensus) cpb2. Atypical beta2-toxin displayed 62.3% identity and 80.4% similarity to consensus beta2-toxin. No porcine type C isolates (n = 16) and only 3.3% of porcine type A isolates (n = 60) carried atypical cpb2 genes. However, 88.5% of nonporcine isolates carried atypical cpb2 (n = 78), but beta2-toxin was not expressed. Almost half of the nonporcine consensus cpb2 genes (44.4%) carried a frameshift mutation (n = 9), resulting in an absence of beta2-toxin expression. These findings strengthen the role of beta2-toxin in the pathogenesis of enteritis in neonatal pigs. However, the identification of apparently nonexpressed, atypical cpb2 genes raises the question of whether this protein plays the same role in enteritis in other animal species.

* Corresponding author. Mailing address: Department of Veterinary Science and Microbiology, University of Arizona, 1117 East Lowell St., Tucson, AZ 85721. Phone: (520) 621-5996. Fax: (520) 621-6366. E-mail: jost{at}u.arizona.edu.

Editor: J. T. Barbieri

Infection and Immunity, January 2005, p. 652-656, Vol. 73, No. 1
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.1.652-656.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Crespo, R., Fisher, D. J., Shivaprasad, H.L., Fernandez-Miyakawa, M. E., Uzal, F. A. (2007). Toxinotypes of Clostridium Perfringens Isolated from Sick and Healthy Avian Species. jvdi 19: 329-333 [Abstract] [Full Text]  
  • Li, J., Miyamoto, K., McClane, B. A. (2007). Comparison of Virulence Plasmids among Clostridium perfringens Type E Isolates. Infect. Immun. 75: 1811-1819 [Abstract] [Full Text]  
  • Miyamoto, K., Fisher, D. J., Li, J., Sayeed, S., Akimoto, S., McClane, B. A. (2006). Complete Sequencing and Diversity Analysis of the Enterotoxin-Encoding Plasmids in Clostridium perfringens Type A Non-Food-Borne Human Gastrointestinal Disease Isolates. J. Bacteriol. 188: 1585-1598 [Abstract] [Full Text]  
  • Harrison, B., Raju, D., Garmory, H. S., Brett, M. M., Titball, R. W., Sarker, M. R. (2005). Molecular Characterization of Clostridium perfringens Isolates from Humans with Sporadic Diarrhea: Evidence for Transcriptional Regulation of the Beta2-Toxin-Encoding Gene. Appl. Environ. Microbiol. 71: 8362-8370 [Abstract] [Full Text]  
  • Sayeed, S., Fernandez-Miyakawa, M. E., Fisher, D. J., Adams, V., Poon, R., Rood, J. I., Uzal, F. A., McClane, B. A. (2005). Epsilon-Toxin Is Required for Most Clostridium perfringens Type D Vegetative Culture Supernatants To Cause Lethality in the Mouse Intravenous Injection Model. Infect. Immun. 73: 7413-7421 [Abstract] [Full Text]  
  • Waters, M., Raju, D., Garmory, H. S., Popoff, M. R., Sarker, M. R. (2005). Regulated Expression of the Beta2-Toxin Gene (cpb2) in Clostridium perfringens Type A Isolates from Horses with Gastrointestinal Diseases. J. Clin. Microbiol. 43: 4002-4009 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»