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Infection and Immunity, October 2005, p. 7027-7031, Vol. 73, No. 10
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.10.7027-7031.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cloning Vectors and Fluorescent Proteins Can Significantly Inhibit Salmonella enterica Virulence in Both Epithelial Cells and Macrophages: Implications for Bacterial Pathogenesis Studies

Leigh A. Knodler,1 Aaron Bestor,1 Caixia Ma,2 Imke Hansen-Wester,3 Michael Hensel,3 Bruce A. Vallance,2 and Olivia Steele-Mortimer1*

Laboratory of Intracellular Parasites, NIAID, NIH, Rocky Mountain Laboratories, Hamilton, Montana,1 Division of Gastroenterology, British Columbia's Children's Hospital and the University of British Columbia, Vancouver, British Columbia, Canada,2 Institut für Klinische Mikrobiologie, Immunologie und Hygiene, Universität Erlangen-Nuernberg, Erlangen, Germany3

Received 31 March 2005/ Returned for modification 31 May 2005/ Accepted 13 June 2005

Plasmid vectors and fluorescent protein reporter systems are commonly used in the study of bacterial pathogenesis. Here we show that they can impair the ability of Salmonella enterica serovar Typhimurium to productively infect either cultured mammalian cells or mice. This has significant implications for studies that rely on these systems.

* Corresponding author. Mailing address: Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT 59840. Phone: (406) 363-9292. Fax: (406) 363-9380. E-mail: omortimer{at}niaid.nih.gov.

Editor: F. C. Fang

Infection and Immunity, October 2005, p. 7027-7031, Vol. 73, No. 10
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.10.7027-7031.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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