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Infection and Immunity, October 2005, p. 7032-7039, Vol. 73, No. 10
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.10.7032-7039.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of Actinobacillus suis Genes Essential for the Colonization of the Upper Respiratory Tract of Swine

Shivani Ojha,1 Marc Sirois,2 and Janet I. MacInnes1*

University of Guelph, Ontario N1G 2W1, Canada,1 Université du Québec à Trois-Rivières, Trois-Rivières, Québec G9A 5H7, Canada2

Received 5 April 2005/ Returned for modification 10 June 2005/ Accepted 29 June 2005

Actinobacillus suis has emerged as an important opportunistic pathogen of high-health-status swine. A colonization challenge method was developed, and using PCR-based signature-tagged transposon mutagenesis, 13 genes belonging to 9 different functional classes were identified that were necessary for A. suis colonization of the upper respiratory tract of swine.

* Corresponding author. Mailing address: Department of Pathobiology, University of Guelph, Guelph, Ontario, Canada N1G 2W1. Phone: (519) 824-4120, ext. 54731. Fax: (519) 824-5930. E-mail: macinnes{at}uoguelph.ca.

Editor: A. D. O'Brien

Infection and Immunity, October 2005, p. 7032-7039, Vol. 73, No. 10
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.10.7032-7039.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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