This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Qazi, K. R.
Right arrow Articles by Fernández, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qazi, K. R.
Right arrow Articles by Fernández, C.

 Previous Article  |  Next Article 

Infection and Immunity, November 2005, p. 7687-7696, Vol. 73, No. 11
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.11.7687-7696.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Exposure to Mycobacteria Primes the Immune System for Evolutionarily Diverse Heat Shock Proteins{dagger}

Khaleda Rahman Qazi,1 Mousumi Rahman Qazi,1 Esther Julián,1 Mahavir Singh,2 Manuchehr Abedi-Valugerdi,1 and Carmen Fernández1*

Department of Immunology, Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden,1 Lionex Diagnostics & Therapeutics, Braunschweig, Germany2

Received 15 March 2005/ Returned for modification 16 May 2005/ Accepted 29 July 2005

During stress conditions, such as infection, the synthesis of heat shock proteins (HSPs) in microorganisms is upregulated. Since a high degree of homology exists within each HSP family, we postulated that exposure to microorganisms could prime the immune system for evolutionarily diverse HSPs. We tested this hypothesis by priming mice with three microorganisms, namely, Mycobacterium bovis BCG, Mycobacterium vaccae, and Chlamydia pneumoniae. After this, mice received a dose of the various HSPs. We found that BCG and M. vaccae but not C. pneumoniae primed the immune system for the induction of secondary immunoglobulin G (IgG) responses to most of the HSPs tested. Analysis of the IgG1 and IgG2a profile and gamma interferon production induced against the HSPs revealed the induction of a mixture of responses. We also observed that sera from mice treated with M. vaccae and HSP70 were cross-reactive, but no antibody complexes were observed in their kidneys, which frequently are targets for autoantibody reactions. Our findings add further support for the use of HSPs as effective vaccine adjuvants.

* Corresponding author. Mailing address: Department of Immunology, Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden. Phone: 46 8 16 45 99. Fax: 46 8 612 95 42. E-mail: carmen.fernandez{at}imun.su.se.

Editor: J. L. Flynn

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Infection and Immunity, November 2005, p. 7687-7696, Vol. 73, No. 11
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.11.7687-7696.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • LaRue, R. W., Dill, B. D., Giles, D. K., Whittimore, J. D., Raulston, J. E. (2007). Chlamydial Hsp60-2 Is Iron Responsive in Chlamydia trachomatis Serovar E-Infected Human Endometrial Epithelial Cells In Vitro. Infect. Immun. 75: 2374-2380 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»