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Infection and Immunity, February 2005, p. 1226-1231, Vol. 73, No. 2
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.2.1226-1231.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Down-Regulation of the kps Region 1 Capsular Assembly Operon following Attachment of Escherichia coli Type 1 Fimbriae to D-Mannose Receptors

William R. Schwan,1* Michael T. Beck,1,{dagger} Scott J. Hultgren,2 Jerry Pinkner,2 Nathan L. Woolever,1,{ddagger} and Thomas Larson1

University of Wisconsin-La Crosse, La Crosse, Wisconsin,1 Washington University, St. Louis, Missouri2

Received 10 August 2004/ Returned for modification 17 September 2004/ Accepted 30 September 2004

A differential-display PCR procedure identified the capsular assembly gene kpsD after Escherichia coli type 1 fimbrial binding to mannose-coated Sepharose beads. Limiting-dilution reverse-transcribed PCRs confirmed down-regulation of the kpsD gene, and Northern blot and lacZ fusion analyses showed down-regulation of the kpsFEDUCS region 1 operon. KpsD protein levels fell, and an agglutination test showed less K capsular antigen on the surface following the bacterial ligand-receptor interaction. These data show that binding of type 1 fimbriae (pili) to D-mannose receptors triggers a cross talk that leads to down-regulation of the capsule assembly region 1 operon in uropathogenic E. coli.

* Corresponding author. Mailing address: Department of Microbiology, University of Wisconsin-La Crosse, 1725 State St., La Crosse, WI 54601. Phone: (608) 785-6980. Fax: (608) 785-6460. E-mail: schwan.will{at}uwlax.edu.

Editor: D. L. Burns

{dagger} Present address: University of Minnesota, Minneapolis, Minn.

{ddagger} Present address: State Lab of Hygiene, Madison, Wis.

Infection and Immunity, February 2005, p. 1226-1231, Vol. 73, No. 2
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.2.1226-1231.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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