This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ibrahim, Y. M.
Right arrow Articles by Mitchell, T. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ibrahim, Y. M.
Right arrow Articles by Mitchell, T. J.

 Previous Article  |  Next Article 

Infection and Immunity, February 2005, p. 730-740, Vol. 73, No. 2
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.2.730-740.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Contribution of the ATP-Dependent Protease ClpCP to the Autolysis and Virulence of Streptococcus pneumoniae

Yasser Musa Ibrahim, Alison R. Kerr, Nuno A. Silva, and Tim J. Mitchell*

Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, United Kingdom

Received 22 June 2004/ Returned for modification 5 August 2004/ Accepted 9 September 2004

The ATP-dependent caseinolytic proteases (Clp) are fundamental for stress tolerance and virulence in many pathogenic bacteria. The role of ClpC in the autolysis and virulence of Streptococcus pneumoniae is controversial. In this study, we tested the role of ClpC in a number of S. pneumoniae strains and found that the contribution of ClpC to autolysis is strain dependent. ClpC is required for the release of autolysin A and pneumolysin in serotype 2 S. pneumoniae strain D39. In vivo, ClpC is required for the growth of the pneumococcus in the lungs and blood in a murine model of disease, but it does not affect the overall outcome of pneumococcal disease. We also report the requirement of ClpP for the growth at elevated temperature and virulence of serotype 4 strain TIGR4 and confirm its contribution to the thermotolerance, oxidative stress resistance, and virulence of D39.

* Corresponding author. Mailing address: Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12-8QQ, United Kingdom. Phone: 44 141 330 3749. Fax: 44 141 3303727. E-mail: t.mitchell{at}bio.gla.ac.uk.

Editor: J. N. Weiser

Infection and Immunity, February 2005, p. 730-740, Vol. 73, No. 2
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.2.730-740.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hendriksen, W. T., Bootsma, H. J., van Diepen, A., Estevao, S., Kuipers, O. P., de Groot, R., Hermans, P. W. M. (2009). Strain-specific impact of PsaR of Streptococcus pneumoniae on global gene expression and virulence. Microbiology 155: 1569-1579 [Abstract] [Full Text]  
  • Kajfasz, J. K., Martinez, A. R., Rivera-Ramos, I., Abranches, J., Koo, H., Quivey, R. G. Jr., Lemos, J. A. (2009). Role of Clp Proteins in Expression of Virulence Properties of Streptococcus mutans. J. Bacteriol. 191: 2060-2068 [Abstract] [Full Text]  
  • de Bruijn, I., Raaijmakers, J. M. (2009). Regulation of Cyclic Lipopeptide Biosynthesis in Pseudomonas fluorescens by the ClpP Protease. J. Bacteriol. 191: 1910-1923 [Abstract] [Full Text]  
  • Zhang, J., Banerjee, A., Biswas, I. (2009). Transcription of clpP Is Enhanced by a Unique Tandem Repeat Sequence in Streptococcus mutans. J. Bacteriol. 191: 1056-1065 [Abstract] [Full Text]  
  • Munoz-Elias, E. J., Marcano, J., Camilli, A. (2008). Isolation of Streptococcus pneumoniae Biofilm Mutants and Their Characterization during Nasopharyngeal Colonization. Infect. Immun. 76: 5049-5061 [Abstract] [Full Text]  
  • Cohn, M. T., Ingmer, H., Mulholland, F., Jorgensen, K., Wells, J. M., Brondsted, L. (2007). Contribution of Conserved ATP-Dependent Proteases of Campylobacter jejuni to Stress Tolerance and Virulence. Appl. Environ. Microbiol. 73: 7803-7813 [Abstract] [Full Text]  
  • Regev-Yochay, G., Trzcinski, K., Thompson, C. M., Lipsitch, M., Malley, R. (2007). SpxB Is a Suicide Gene of Streptococcus pneumoniae and Confers a Selective Advantage in an In Vivo Competitive Colonization Model. J. Bacteriol. 189: 6532-6539 [Abstract] [Full Text]  
  • Tu, L. N., Jeong, H.-Y., Kwon, H.-Y., Ogunniyi, A. D., Paton, J. C., Pyo, S.-N., Rhee, D.-K. (2007). Modulation of Adherence, Invasion, and Tumor Necrosis Factor Alpha Secretion during the Early Stages of Infection by Streptococcus pneumoniae ClpL. Infect. Immun. 75: 2996-3005 [Abstract] [Full Text]  
  • Lanie, J. A., Ng, W.-L., Kazmierczak, K. M., Andrzejewski, T. M., Davidsen, T. M., Wayne, K. J., Tettelin, H., Glass, J. I., Winkler, M. E. (2007). Genome Sequence of Avery's Virulent Serotype 2 Strain D39 of Streptococcus pneumoniae and Comparison with That of Unencapsulated Laboratory Strain R6. J. Bacteriol. 189: 38-51 [Abstract] [Full Text]  
  • DelVecchio, V. G., Connolly, J. P., Alefantis, T. G., Walz, A., Quan, M. A., Patra, G., Ashton, J. M., Whittington, J. T., Chafin, R. D., Liang, X., Grewal, P., Khan, A. S., Mujer, C. V. (2006). Proteomic Profiling and Identification of Immunodominant Spore Antigens of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis. Appl. Environ. Microbiol. 72: 6355-6363 [Abstract] [Full Text]  
  • Graham, M. R., Virtaneva, K., Porcella, S. F., Gardner, D. J., Long, R. D., Welty, D. M., Barry, W. T., Johnson, C. A., Parkins, L. D., Wright, F. A., Musser, J. M. (2006). Analysis of the Transcriptome of Group A Streptococcus in Mouse Soft Tissue Infection. Am. J. Pathol. 169: 927-942 [Abstract] [Full Text]  
  • Henderson, B., Allan, E., Coates, A. R. M. (2006). Stress Wars: the Direct Role of Host and Bacterial Molecular Chaperones in Bacterial Infection. Infect. Immun. 74: 3693-3706 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»