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Infection and Immunity, April 2005, p. 2541-2546, Vol. 73, No. 4
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.4.2541-2546.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Antibody against the Carboxyl Terminus of Intimin {alpha} Reduces Enteropathogenic Escherichia coli Adherence to Tissue Culture Cells and Subsequent Induction of Actin Polymerization

Humberto M. Carvalho, Louise D. Teel, John F. Kokai-Kun,1,{dagger} and Alison D. O'Brien1*

Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland

Received 13 October 2004/ Returned for modification 18 November 2004/ Accepted 24 November 2004

The C-terminal third of intimin binds to its translocated receptor (Tir) to promote attaching and effacing lesion formation during infection with enteropathogenic Escherichia coli (EPEC). We observed that the adherence of EPEC strains to HEp-2 cells was reduced and that actin polymerization was blocked by antibody raised against the C-terminal third of intimin {alpha}.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, B4052, 4301 Jones Bridge Rd., Bethesda, MD 20814-4799. Phone: (301) 295-3400. Fax: (301) 295-3773. E-mail: aobrien{at}usuhs.mil.

Editor: J. T. Barbieri

{dagger} Present address: Biosynexus Inc., Gaithersburg, MD 20877.

Infection and Immunity, April 2005, p. 2541-2546, Vol. 73, No. 4
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.4.2541-2546.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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