This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maeda, Y.
Right arrow Articles by Makino, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maeda, Y.
Right arrow Articles by Makino, M.

 Previous Article  |  Next Article 

Infection and Immunity, May 2005, p. 2744-2750, Vol. 73, No. 5
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.5.2744-2750.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of an Immunomodulating Agent from Mycobacterium leprae

Yumi Maeda,1 Tetsu Mukai,1 John Spencer,2 and Masahiko Makino1*

Department of Microbiology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aobacho, Higashimurayama, Tokyo 189-0002, Japan,1 Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523-16772

Received 22 October 2004/ Returned for modification 1 December 2004/ Accepted 7 January 2005

A search for an immunomodulating agent from mycobacteria was carried out using Mycobacterium leprae. The antigenicity of each fraction of the bacterial membrane, which contains the most antigenic components of M. leprae, was assessed by using sera from paucibacillary leprosy. N-terminal sequencing of the serum-reactive protein and functional assessment of the membrane fractions using monocyte-derived dendritic cells (DCs) identified major membrane protein II (MMP-II) as one of the efficient T-cell-activating candidates. Purified MMP-II stimulated DCs from healthy individuals to produce interleukin-12 p70 and up-regulated the surface expression of major histocompatibility complex class I and II, CD86, and CD83 molecules. Also, there was an increase in the percentage of CD83+ cells in the DC population. Furthermore, MMP-II-pulsed DCs expressed their derivatives on their surfaces. Using Toll-like receptor 2 (TLR-2)-dependent receptor constructs, we found that TLR-2 signaling was involved in DC maturation induced by MMP-II. Taken together, MMP-II can be recognized as an immunomodulating protein in terms of activation of antigen-presenting cells and innate immunity.

* Corresponding author. Mailing address: Department of Microbiology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aobacho, Higashimurayama, Tokyo 189-0002, Japan. Phone: 81-42-391-8059. Fax: 81-42-391-8212. E-mail: mmaki{at}nih.go.jp.

Editor: W. A. Petri, Jr.

Infection and Immunity, May 2005, p. 2744-2750, Vol. 73, No. 5
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.5.2744-2750.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mukai, T., Maeda, Y., Tamura, T., Matsuoka, M., Tsukamoto, Y., Makino, M. (2009). Induction of Cross-Priming of Naive CD8+ T Lymphocytes by Recombinant Bacillus Calmette-Guerin That Secretes Heat Shock Protein 70-Major Membrane Protein-II Fusion Protein. J. Immunol. 183: 6561-6568 [Abstract] [Full Text]  
  • Maeda, Y., Tamura, T., Matsuoka, M., Makino, M. (2009). Inhibition of the Multiplication of Mycobacterium leprae by Vaccination with a Recombinant M. bovis BCG Strain That Secretes Major Membrane Protein II in Mice. CVI 16: 1399-1404 [Abstract] [Full Text]  
  • Kai, M., Nguyen Phuc, N. H., Hoang Thi, T. H., Nguyen, A. H., Fukutomi, Y., Maeda, Y., Miyamoto, Y., Mukai, T., Fujiwara, T., Nguyen, T. T., Makino, M. (2008). Serological Diagnosis of Leprosy in Patients in Vietnam by Enzyme-Linked Immunosorbent Assay with Mycobacterium leprae-Derived Major Membrane Protein II. CVI 15: 1755-1759 [Abstract] [Full Text]  
  • Murray, R. A., Siddiqui, M. R., Mendillo, M., Krahenbuhl, J., Kaplan, G. (2007). Mycobacterium leprae Inhibits Dendritic Cell Activation and Maturation. J. Immunol. 178: 338-344 [Abstract] [Full Text]  
  • Makino, M., Maeda, Y., Inagaki, K. (2006). Immunostimulatory Activity of Recombinant Mycobacterium bovis BCG That Secretes Major Membrane Protein II of Mycobacterium leprae.. Infect. Immun. 74: 6264-6271 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»