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Infection and Immunity, May 2005, p. 2848-2856, Vol. 73, No. 5
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.5.2848-2856.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cross-Reactive Surface Epitopes on Chondroitin Sulfate A-Adherent Plasmodium falciparum-Infected Erythrocytes Are Associated with Transcription of var2csa

Salenna R. Elliott,* Michael F. Duffy, Timothy J. Byrne, James G. Beeson,{dagger} Emily J. Mann, Danny W. Wilson,{dagger} Stephen J. Rogerson, and Graham V. Brown

Department of Medicine, University of Melbourne, Royal Melbourne Hospital, Victoria, Australia

Received 18 November 2004/ Returned for modification 30 December 2004/ Accepted 7 January 2005

Malaria in pregnancy is associated with placental accumulation of Plasmodium falciparum-infected erythrocytes (IE) that adhere to chondroitin sulfate A (CSA). Adhesion is mediated by P. falciparum erythrocyte membrane protein 1 (PfEMP1), a variant parasite protein expressed on the surface of IE and encoded by var genes. Rabbit antiserum was generated against the CSA-adherent P. falciparum line CS2, in which the dominant var transcribed is var2csa, a relatively conserved var gene that has been associated with CSA adhesion. Anti-CS2 recognized genetically distinct CSA-adherent P. falciparum lines but not CD36-adherent parent lines. Reactivity with anti-CS2 correlated with the level of adhesion to CSA. Fluorescence-activated cell sorting according to binding of anti-CS2 showed reactivity was associated with CSA adhesion and transcription of var2csa. These data are consistent with the hypothesis that var2csa encodes a PfEMP1 expressed on the surface of IE, which mediates adhesion to CSA and is relatively conserved between genetically distinct strains of P. falciparum.

* Corresponding author. Mailing address: Department of Medicine, University of Melbourne, Royal Melbourne Hospital, Victoria 3050 Australia. Phone: 613 8344 3267. Fax: 613 9347 1863. E-mail: salenna{at}unimelb.edu.au.

Editor: W. A. Petri, Jr.

{dagger} Present address: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.

Infection and Immunity, May 2005, p. 2848-2856, Vol. 73, No. 5
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.5.2848-2856.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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