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Infection and Immunity, June 2005, p. 3794-3798, Vol. 73, No. 6
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.6.3794-3798.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genomic Comparison of cag Pathogenicity Island (PAI)-Positive and -Negative Helicobacter pylori Strains: Identification of Novel Markers for cag PAI-Positive Strains

Courtney E. Terry,1,{dagger} Lisa M. McGinnis,1 Katharine C. Madigan,1,{ddagger} Ping Cao,2 Timothy L. Cover,2,4 George W. Liechti,1 Richard M. Peek Jr.,3,4 and Mark H. Forsyth1*

The College of William and Mary, Department of Biology, Williamsburg, Virginia 23187-8795,1 Vanderbilt University School of Medicine, Division of Infectious Diseases, Departments of Medicine and Microbiology and Immunology, Nashville, Tennessee 37232-2279,2 Vanderbilt University School of Medicine, Department of Medicine, Division of Gastroenterology, and Department of Cancer Biology, Nashville, Tennessee 37232-2279,3 Department of Veterans Affairs Medical Center, Nashville, Tennessee 372124

Received 22 September 2004/ Returned for modification 20 December 2004/ Accepted 7 February 2005

In an analysis of Helicobacter pylori genomic DNA by macroarray methodology, genomic DNA from a panel of cag pathogenicity island (PAI)-negative H. pylori clinical isolates failed to hybridize with 27 genes located outside the cag PAI in a cag PAI-positive reference strain. PCR analyses confirmed that HP0217 (encoding a lipopolysaccharide biosynthetic protein) and HP1079 (encoding a protein of unknown function) were present significantly more frequently in cagA-positive strains than in cagA-negative strains. A low G+C content of these two genes suggests they were acquired by horizontal transfer events.

* Corresponding author. Mailing address: Department of Biology, The College of William & Mary, 214 Millington Hall, Landrum Drive, Williamsburg, VA 23187-8795. Phone: (757) 221-2489. Fax: (757) 221-6483. E-mail: mhfors{at}wm.edu.

Editor: V. J. DiRita

{dagger} Present address: University of Virginia School of Medicine, Charlottesville, VA 22908.

{ddagger} Present address: Thomas Jefferson University, Jefferson Medical College, Philadelphia, PA 19107.

Infection and Immunity, June 2005, p. 3794-3798, Vol. 73, No. 6
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.6.3794-3798.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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