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Infection and Immunity, July 2005, p. 4062-4069, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.4062-4069.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Protection against Leptospira interrogans Sensu Lato Challenge by DNA Immunization with the Gene Encoding Hemolysin-Associated Protein 1

C. Branger,1,{dagger} B. Chatrenet,2 A. Gauvrit,3 F. Aviat,1 A. Aubert,2 J. M. Bach,3 and G. André-Fontaine1*

Leptospira Medical and Molecular Bacteriology Unit, Ecole Nationale Vétérinaire de Nantes, BP 40706, 44307 Nantes cedex 03, France,1 Virbac Laboratories, BP 27, 06511 Carros Cedex, France,2 Immuno-endocrinology Unit, ENVN/INRA/University, BP 40706, 44307 Nantes cedex 03, France3

Received 4 October 2004/ Returned for modification 17 November 2004/ Accepted 3 March 2005

The use of DNA constructs encoding leptospiral proteins is a promising new approach for vaccination against leptospirosis. In previous work we determined that immunization with hemolysis-associated protein 1 (Hap1) (LipL32) expressed by adenovirus induced significant protection against a virulent Leptospira challenge in gerbils. To avoid the use of the adenovirus vector, we checked for clinical protection against lethal challenge by DNA vaccination. A DNA vaccine expressing Hap1 was designed to enhance the direct gene transfer of this protein into gerbils. A challenge was performed 3 weeks after the last immunization with a virulent strain of serovar canicola. Our results show that the cross-protective effect with pathogenic strains of Leptospira, shared by Hap1, could be mediated by the DNA plasmid vector. This finding should facilitate the design and development of a new generation of vaccines against bacteria, particularly Leptospira interrogans sensu lato.

* Corresponding author. Mailing address: Unité de Bactériologie Médicale et Moléculaire des Leptospires, Ecole Nationale Vétérinaire de Nantes, BP 40706, 44307 Nantes cedex 03, France. Phone: 33 240 68 76 91. Fax: 33 240 68 76 94. E-mail: andre-fontaine{at}vet-nantes.fr.

Editor: J. B. Bliska

{dagger} Present address: Department of Biology, Washington University, 1 Brookings Dr., Campus Box 1137, St. Louis, MO 63130.

Infection and Immunity, July 2005, p. 4062-4069, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.4062-4069.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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