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Infection and Immunity, July 2005, p. 4420-4422, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.4420-4422.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Complement Depletion Renders C57BL/6 Mice Sensitive to the Bacillus anthracis Sterne Strain

Eric T. Harvill,2 Gloria Lee,1 Vanessa K. Grippe,1 and Tod J. Merkel1*

Laboratory of Respiratory and Special Pathogens, Center for Biologics Evaluation and Research, Food and Drug Administration, 8800 Rockville Pike, Bethesda, Maryland 20892,1 Immunology Research Laboratories, Department of Veterinary Science, The Pennsylvania State University, University Park, Pennsylvania2

Received 18 October 2004/ Returned for modification 10 January 2005/ Accepted 16 February 2005

Concerns regarding safety and control of virulent Bacillus anthracis have created substantial hurdles to the study of anthrax. The Sterne strain is considered relatively safe to study, but this acapsular strain has a defect in normal mice and is often studied in A/J mice. A/J mice are highly susceptible to the Sterne strain, due to a defect in the Hc locus, which encodes complement factor 5 (C5). Here we show that normally resistant C57BL/6 mice become highly susceptible to the Sterne strain upon complement depletion with cobra venom factor. This generalizable approach should allow the virulence of anthrax to be studied under relatively safe conditions and using a wide variety of mouse strains.

* Corresponding author. Mailing address: Laboratory of Respiratory and Special Pathogens, DBPAP/CBER/FDA, Building 29, Room 418, 29 Lincoln Drive, Bethesda, MD 20892. Phone: (301) 496-5564. Fax: (301) 402-2776. E-mail: merkel{at}cber.fda.gov.

Editor: A. D. O'Brien

Infection and Immunity, July 2005, p. 4420-4422, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.4420-4422.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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