This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rouabhia, M.
Right arrow Articles by Ernst, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rouabhia, M.
Right arrow Articles by Ernst, J. F.

 Previous Article  |  Next Article 

Infection and Immunity, August 2005, p. 4571-4580, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.4571-4580.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Virulence of the Fungal Pathogen Candida albicans Requires the Five Isoforms of Protein Mannosyltransferases

Mahmoud Rouabhia,2 Martin Schaller,3 Cristina Corbucci,4 Anna Vecchiarelli,4 Stephan K.-H. Prill,1 Luc Giasson,1,2 and Joachim F. Ernst1*

Institut für Mikrobiologie, Heinrich-Heine-Universität, Düsseldorf, Germany,1 Faculté de médecine dentaire, Université Laval, Québec, Canada,2 Universitäts-Hautklinik, Klinikum der Eberhard Karls Universität Tübingen, Tübingen, Germany,3 Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Universita degli Studi di Perugia, Perugia, Italy4

Received 22 September 2004/ Returned for modification 13 October 2004/ Accepted 14 March 2005

The PMT gene family in Candida albicans encodes five isoforms of protein mannosyltransferases (Pmt proteins Pmt1p, Pmt2p, Pmt4p, Pmt5p, and Pmt6p) that initiate O mannosylation of secretory proteins. We compared virulence characteristics of pmt mutants in two complex, three-dimensional models of localized candidiasis, using reconstituted human epithelium (RHE) and engineered human oral mucosa (EHOM); in addition, mutants were tested in a mouse model of hematogenously disseminated candidiasis (HDC). All pmt mutants showed attenuated virulence in the HDC model and at least one model of localized candidiasis. The pmt5 mutant, which lacks in vitro growth phenotypes, was less virulent in the EHOM and HDC assays but had no consistent phenotype in the RHE assay. In contrast, the pmt4 and pmt6 mutants were less virulent in the RHE and HDC assays but not in the EHOM assay. The results stress the contribution of all Pmt isoforms to the virulence of C. albicans and suggest that the importance of individual Pmt isoforms may differ in specific host niches. We propose that Pmt proteins may be suitable targets for future novel classes of antifungal agents.

* Corresponding author. Mailing address: Institut for Mikrobiologie, Universitätsstr. 1/26.12, 40225 Düsseldorf, Germany. Phone and fax: 49(211)811-5176. E-mail: Joachim.ernst{at}uni-duesseldorf.de.

Editor: T. R. Kozel

Infection and Immunity, August 2005, p. 4571-4580, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.4571-4580.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kriangkripipat, T., Momany, M. (2009). Aspergillus nidulans Protein O-Mannosyltransferases Play Roles in Cell Wall Integrity and Developmental Patterning. Eukaryot Cell 8: 1475-1485 [Abstract] [Full Text]  
  • Mille, C., Bobrowicz, P., Trinel, P.-A., Li, H., Maes, E., Guerardel, Y., Fradin, C., Martinez-Esparza, M., Davidson, R. C., Janbon, G., Poulain, D., Wildt, S. (2008). Identification of a New Family of Genes Involved in {beta}-1,2-Mannosylation of Glycans in Pichia pastoris and Candida albicans. J. Biol. Chem. 283: 9724-9736 [Abstract] [Full Text]  
  • Brand, A., Vacharaksa, A., Bendel, C., Norton, J., Haynes, P., Henry-Stanley, M., Wells, C., Ross, K., Gow, N. A. R., Gale, C. A. (2008). An Internal Polarity Landmark Is Important for Externally Induced Hyphal Behaviors in Candida albicans. Eukaryot Cell 7: 712-720 [Abstract] [Full Text]  
  • Mora-Montes, H. M., Bates, S., Netea, M. G., Diaz-Jimenez, D. F., Lopez-Romero, E., Zinker, S., Ponce-Noyola, P., Kullberg, B. J., Brown, A. J. P., Odds, F. C., Flores-Carreon, A., Gow, N. A. R. (2007). Endoplasmic Reticulum {alpha}-Glycosidases of Candida albicans Are Required for N Glycosylation, Cell Wall Integrity, and Normal Host-Fungus Interaction. Eukaryot Cell 6: 2184-2193 [Abstract] [Full Text]  
  • Olson, G. M., Fox, D. S., Wang, P., Alspaugh, J. A., Buchanan, K. L. (2007). Role of Protein O-Mannosyltransferase Pmt4 in the Morphogenesis and Virulence of Cryptococcus neoformans. Eukaryot Cell 6: 222-234 [Abstract] [Full Text]  
  • Peltroche-Llacsahuanga, H., Goyard, S., d'Enfert, C., Prill, S. K.-H., Ernst, J. F. (2006). Protein O-Mannosyltransferase Isoforms Regulate Biofilm Formation in Candida albicans.. Antimicrob. Agents Chemother. 50: 3488-3491 [Abstract] [Full Text]  
  • Bai, C., Xu, X.-L., Chan, F.-Y., Lee, R. T. H., Wang, Y. (2006). MNN5 Encodes an Iron-Regulated {alpha}-1,2-Mannosyltransferase Important for Protein Glycosylation, Cell Wall Integrity, Morphogenesis, and Virulence in Candida albicans. Eukaryot Cell 5: 238-247 [Abstract] [Full Text]  
  • Bates, S., Hughes, H. B., Munro, C. A., Thomas, W. P. H., MacCallum, D. M., Bertram, G., Atrih, A., Ferguson, M. A. J., Brown, A. J. P., Odds, F. C., Gow, N. A. R. (2006). Outer Chain N-Glycans Are Required for Cell Wall Integrity and Virulence of Candida albicans. J. Biol. Chem. 281: 90-98 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»