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Infection and Immunity, August 2005, p. 5183-5188, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.5183-5188.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Probiotics Reduce Enterohemorrhagic Escherichia coli O157:H7- and Enteropathogenic E. coli O127:H6-Induced Changes in Polarized T84 Epithelial Cell Monolayers by Reducing Bacterial Adhesion and Cytoskeletal Rearrangements

Philip M. Sherman,1* Kathene C. Johnson-Henry,1 Helen P. Yeung,1 Peter S. C. Ngo,1 Jacques Goulet,2 and Thomas A. Tompkins3

Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario,1 Laval University, Quebec City, Quebec,2 Institut Rosell-Lallemand Inc., Montreal, Quebec, Canada3

Received 9 October 2004/ Returned for modification 4 January 2005/ Accepted 6 April 2005

The aim of this study was to determine if probiotics reduce epithelial injury following exposure to Escherichia coli O157:H7 and E. coli O127:H6. The pretreatment of intestinal (T84) cells with lactic acid-producing bacteria reduced the pathogen-induced drop in transepithelial electrical resistance. These findings demonstrate that probiotics prevent epithelial injury induced by attaching-effacing bacteria.

* Corresponding author. Mailing address: Gastroenterology & Nutrition, Room 8409, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada. Phone: (416) 813-7734. Fax: (416) 813-6531. E-mail: sherman{at}sickkids.ca.

Editor: V. J. DiRita

Infection and Immunity, August 2005, p. 5183-5188, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.5183-5188.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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