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Infection and Immunity, August 2005, p. 5256-5261, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.5256-5261.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Protection against Aerosolized Yersinia pestis Challenge following Homologous and Heterologous Prime-Boost with Recombinant Plague Antigens

Audrey Glynn,1,{dagger} Chad J. Roy,2,{dagger} Bradford S. Powell,2 Jeffrey J. Adamovicz,2 Lucy C. Freytag,1 and John D. Clements1*

Department of Microbiology and Immunology, Program in Molecular Pathogenesis and Immunity, Tulane University Health Sciences Center, New Orleans, Louisiana 70112,1 Division of Bacteriology, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 217022

Received 14 January 2005/ Returned for modification 15 February 2005/ Accepted 16 March 2005

A Yersinia pestis-derived fusion protein (F1-V) has shown great promise as a protective antigen against aerosol challenge with Y. pestis in murine studies. In the current study, we examined different prime-boost regimens with F1-V and demonstrate that (i) boosting by a route other than the route used for the priming dose (heterologous boosting) protects mice as well as homologous boosting against aerosol challenge with Y. pestis, (ii) parenteral immunization is not required to protect mice against aerosolized plague challenge, (iii) the route of immunization and choice of adjuvant influence the magnitude of the antibody response as well as the immunoglobulin G1 (IgG1)/IgG2a ratio, and (iv) inclusion of an appropriate adjuvant is critical for nonparenteral immunization.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Program in Molecular Pathogenesis and Immunity, 1430 Tulane Avenue, Tulane University Health Sciences Center, New Orleans, LA 70112. Phone: (504) 988-5070. Fax: (504) 988-5144. E-mail: jclemen{at}tulane.edu.

Editor: D. L. Burns

{dagger} A.G. and C.J.R. contributed equally to this work.

Infection and Immunity, August 2005, p. 5256-5261, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.5256-5261.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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