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Infection and Immunity, September 2005, p. 5426-5437, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.5426-5437.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cable Pili and the 22-Kilodalton Adhesin Are Required for Burkholderia cenocepacia Binding to and Transmigration across the Squamous Epithelium

Teresa A. Urban,1 Joanna B. Goldberg,1 Janet F. Forstner,2 and Umadevi S. Sajjan2*

Department of Microbiology, University of Virginia Health Sciences, Charlottesville, Virginia,1 Structural Biology and Biochemistry, The Hospital for Sick Children, Toronto, Ontario, Canada2

Received 4 February 2005/ Returned for modification 13 April 2005/ Accepted 4 May 2005

Burkholderia cenocepacia strains expressing both cable (Cbl) pili and the 22-kDa adhesin bind to cytokeratin 13 (CK13) strongly and invade squamous epithelium efficiently. It has not been established, however, whether the gene encoding the adhesin is located in the cbl operon or what specific contribution the adhesin and Cbl pili lend to binding and transmigration or invasion capacity of B. cenocepacia. By immunoscreening an expression library of B. cenocepacia isolate BC7, we identified a large gene (adhA) that encodes the 22-kDa adhesin. Isogenic mutants lacking expression of either Cbl pili (cblA or cblS mutants) or the adhesin (adhA mutant) were constructed to assess the individual role of Cbl pili and the adhesin in mediating B. cenocepacia binding to and transmigration across squamous epithelium. Relative to the parent strain, mutants of Cbl pili showed reduced binding (50%) to isolated CK13, while the adhesin mutant showed almost no binding (0 to 8%). Mutants lacking either cable pili or the adhesin were compromised in their ability to bind to and transmigrate across the squamous epithelium compared to the wild-type strain, although this deficiency was most pronounced in the adhA mutant. These results indicate that both Cbl pili and the 22-kDa adhesin are necessary for the optimal binding to CK13 and transmigration properties of B. cenocepacia.

* Corresponding author. Present address: Department of Pediatrics, Infectious Diseases, University of Michigan, 8323 MSRB III, 1150 W. Medical Center Drive, Ann Arbor, MI 48109. Phone: (734) 936-9767. Fax: (734) 764-4279. E-mail: usajjan{at}umich.edu.

Editor: J. N. Weiser

Infection and Immunity, September 2005, p. 5426-5437, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.5426-5437.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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