This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rosenkrands, I.
Right arrow Articles by Andersen, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rosenkrands, I.
Right arrow Articles by Andersen, P.

 Previous Article  |  Next Article 

Infection and Immunity, September 2005, p. 5817-5826, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.5817-5826.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cationic Liposomes Containing Mycobacterial Lipids: a New Powerful Th1 Adjuvant System

Ida Rosenkrands,{dagger} Else Marie Agger,*{dagger} Anja W. Olsen, Karen S. Korsholm, Claire Swetman Andersen, Klaus T. Jensen, and Peter Andersen

Statens Serum Institut, Department of Infectious Disease Immunology, Copenhagen, Denmark

Received 22 December 2004/ Returned for modification 20 February 2005/ Accepted 18 April 2005

The immunostimulation provided by the mycobacterial cell wall has been exploited for many decades, e.g., in Freund's complete adjuvant. Recently, the underlying mechanism behind this adjuvant activity, including Toll receptor signaling, has begun to be unraveled, confirming the potential of mycobacterial constituents to act as adjuvants. In this study, the immunostimulatory properties of a Mycobacterium bovis BCG lipid extract were tested for their adjuvant activity. Administration of the lipids in dimethyl dioctadecyl ammonium bromide-based cationic liposomes induced a powerful Th1 response characterized by markedly elevated antigen-specific immunoglobulin G2a (IgG2a) isotype antibodies and substantial production of gamma interferon. The adjuvant formulation (designated mycosomes) elicited high levels of gamma interferon both in C57BL/6 as well as in Th2-prone BALB/c mice. Furthermore, the mycosomes induced immune responses to protein antigens from several sources including Mycobacterium tuberculosis, Chlamydia muridarum, and tetanus toxoid. In a tuberculosis challenge model, the mycosomes combined with the Ag85B-ESAT-6 fusion protein were demonstrated to have a unique ability to maintain sustained immunological memory at a level superior to live BCG.

* Corresponding author. Mailing address: Statens Serum Institut, Department of Infectious Disease Immunology, Adjuvant Research, 5 Artillerivej, DK-2300 Copenhagen S, Denmark. Phone: 45 32 68 39 41. Fax: 45 32 68 30 35. E-mail: eag{at}ssi.dk.

Editor: J. D. Clements

{dagger} I.R. and E.A.G. contributed equally to this work.

Infection and Immunity, September 2005, p. 5817-5826, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.5817-5826.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Vordermeier, H. M., Dean, G. S., Rosenkrands, I., Agger, E. M., Andersen, P., Kaveh, D. A., Hewinson, R. G., Hogarth, P. J. (2009). Adjuvants Induce Distinct Immunological Phenotypes in a Bovine Tuberculosis Vaccine Model. CVI 16: 1443-1448 [Abstract] [Full Text]  
  • Andersen, C. A. S., Rosenkrands, I., Olsen, A. W., Nordly, P., Christensen, D., Lang, R., Kirschning, C., Gomes, J. M., Bhowruth, V., Minnikin, D. E., Besra, G. S., Follmann, F., Andersen, P., Agger, E. M. (2009). Novel Generation Mycobacterial Adjuvant Based on Liposome-Encapsulated Monomycoloyl Glycerol from Mycobacterium bovis Bacillus Calmette-Guerin. J. Immunol. 183: 2294-2302 [Abstract] [Full Text]  
  • Lindenstrom, T., Agger, E. M., Korsholm, K. S., Darrah, P. A., Aagaard, C., Seder, R. A., Rosenkrands, I., Andersen, P. (2009). Tuberculosis Subunit Vaccination Provides Long-Term Protective Immunity Characterized by Multifunctional CD4 Memory T Cells. J. Immunol. 182: 8047-8055 [Abstract] [Full Text]  
  • Andersen, C. S., Agger, E. M., Rosenkrands, I., Gomes, J. M., Bhowruth, V., Gibson, K. J. C., Petersen, R. V., Minnikin, D. E., Besra, G. S., Andersen, P. (2009). A Simple Mycobacterial Monomycolated Glycerol Lipid Has Potent Immunostimulatory Activity. J. Immunol. 182: 424-432 [Abstract] [Full Text]  
  • Bastian, M., Braun, T., Bruns, H., Rollinghoff, M., Stenger, S. (2008). Mycobacterial Lipopeptides Elicit CD4+ CTLs in Mycobacterium tuberculosis-Infected Humans. J. Immunol. 180: 3436-3446 [Abstract] [Full Text]  
  • Andersen, C. S., Dietrich, J., Agger, E. M., Lycke, N. Y., Lovgren, K., Andersen, P. (2007). The Combined CTA1-DD/ISCOMs Vector Is an Effective Intranasal Adjuvant for Boosting Prior Mycobacterium bovis BCG Immunity to Mycobacterium tuberculosis. Infect. Immun. 75: 408-416 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»