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Infection and Immunity, September 2005, p. 5908-5914, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.5908-5914.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Transforming Growth Factor ß1 Production by CD4⁺ CD25⁺ Regulatory T Cells in Peripheral Blood Mononuclear Cells from Healthy Subjects Stimulated with Leishmania guyanensis

A. Kariminia,^1, E. Bourreau,¹ H. Pascalis,^1,2 P. Couppié,³ D. Sainte-Marie,³ F. Tacchini-Cottier,⁴ and P. Launois^1,4*

Immunologie des Leishmanioses, Institut Pasteur de la Guyane, 97306 Cayenne,¹ Institut d'Enseignement Supérieur de la Guyane, Université Antilles-Guyane, Cayenne,² Institut Guyanais de Dermatologie Tropicale, E.A. 2188, Centre Hospitalier Andrée Rosemon, 97300, Cayenne, French Guyana,³ WHO-IRTC, Department of Biochemistry, University of Lausanne, Epalinges, Switzerland⁴

Received 6 December 2004/ Returned for modification 20 January 2005/ Accepted 8 May 2005

Transforming growth factor ß (TGF-ß) has been shown to be a central immunomodulator used by leishmaniae to escape effective mechanisms of protection in human and murine infections with these parasites. However, all the information is derived from studies of established infection, while little is known about TGF-ß production in response to Leishmania stimulation in healthy subjects. In this study, TGF-ß1 production was demonstrated in peripheral blood mononuclear cells from healthy subjects never exposed to leishmaniae in response to live Leishmania guyanensis, and the TGF-ß1-producing cells were described as a distinct subpopulation of CD4⁺ CD25⁺ regulatory T cells. The suppressive properties of CD4⁺ CD25⁺ T cells were demonstrated in vitro by their inhibition of production of interleukin 2 (IL-2) and IL-10 by CD4⁺ CD25^– T cells in the presence of either anti-CD3 or L. guyanensis. Although neutralization of TGF-ß1 did not reverse the suppressive activity of CD4⁺ CD25⁺ T cells activated by anti-CD3, it reversed the suppressive activity of CD4⁺ CD25⁺ T cells activated by L. guyanensis. Altogether our data demonstrated that TGF-ß1 is involved in the suppressive activity of L. guyanensis-stimulated CD4⁺ CD25⁺ T cells from healthy controls.

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* Corresponding author. Mailing address: WHO-IRTC, Department of Biochemistry, University of Lausanne, Ch des Boveresses, 155, 1066 Epalinges, Switzerland. Phone: 41 21 692 57 03. Fax: 41 21 692 57 05. E-mail: pascal.launois{at}unil.ib.ch.

Editor: J. F. Urban, Jr.

Present address: Pasteur Institute of Iran, P.O. Box 11365-6699, Tehran, Iran.

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Infection and Immunity, September 2005, p. 5908-5914, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.5908-5914.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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• Bourreau, E., Ronet, C., Darcissac, E., Lise, M. C., Sainte Marie, D., Clity, E., Tacchini-Cottier, F., Couppie, P., Launois, P. (2009). Intralesional Regulatory T-Cell Suppressive Function during Human Acute and Chronic Cutaneous Leishmaniasis Due to Leishmania guyanensis. Infect. Immun. 77: 1465-1474 [Abstract] [Full Text]