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Infection and Immunity, September 2005, p. 6005-6016, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.6005-6016.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Interaction of Enteropathogenic and Shiga Toxin-Producing Escherichia coli and Porcine Intestinal Mucosa: Role of Intimin and Tir in Adherence

Francis Girard,1 Isabelle Batisson,1,2 Gad M. Frankel,3 Josée Harel,1 and John M. Fairbrother1*

Groupe de Recherche sur les Maladies Infectieuses du Porc, Département de Pathologie et Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Canada,1 Laboratoire de Biologie des Protistes, UMR 6023, Université Blaise Pascal, Clermont-II, Aubière, France,2 Center for Molecular Microbiology and Infection, Imperial College London, London, United Kingdom3

Received 28 February 2005/ Returned for modification 7 April 2005/ Accepted 20 April 2005

The ileal in vitro organ culture (IVOC) model using tissues originating from colostrum-deprived newborn piglets has proven to be an effective way to study the attaching and effacing (A/E) phenotype of porcine enteropathogenic Escherichia coli (EPEC) ex vivo. The aim of this study was to investigate the role of intimin subtype and Tir in the adherence of EPEC and Shiga-toxin-producing E. coli (STEC), isolated from different animal species, to porcine intestinal IVOC. Moreover, the role of intimin in Tir-independent adherence of the human EPEC strain E2348/69 was investigated using intimin and Tir-deficient derivatives. Our results demonstrated that A/E E. coli strains (AEEC) from various animal species and humans induce the A/E phenotype in porcine ileal IVOC and that intimin subtype influences intestinal adherence and tropism of AEEC strains. We also showed that a tir mutant of EPEC strain E2348/69 demonstrates close adherence to the epithelial cells of porcine ileal IVOC segments, with microvillous effacement but with no evidence of actin polymerization or pedestal formation, and that intimin seems to be involved in this phenotype. Overall, this study provides further evidence for the existence of one or more host-cell-encoded intimin receptor(s) in the pig gut.

* Corresponding author. Mailing address: Groupe de Recherche sur les Maladies Infectieuses du Porc, Département de Pathologie et Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Canada. Phone: 1 (450) 773 8521, ext. 18234. Fax: 1 (450) 778 8108. E-mail: john.morris.fairbrother{at}umontreal.ca.

Editor: A. D. O'Brien

Infection and Immunity, September 2005, p. 6005-6016, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.6005-6016.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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