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Infection and Immunity, January 2006, p. 265-272, Vol. 74, No. 1
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.1.265-272.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The lpf Gene Cluster for Long Polar Fimbriae Is Not Involved in Adherence of Enteropathogenic Escherichia coli or Virulence of Citrobacter rodentium

Ichiro Tatsuno,1 Rosanna Mundy,2 Gad Frankel,2 Yuwen Chong,3 Alan D. Phillips,3 Alfredo G. Torres,4 and James B. Kaper1*

Center for Vaccine Development and Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, 21201,1 Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555,4 Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ,2 Centre for Paediatric Gastroenterology, Royal Free Hospital, London NW3 2QG, United Kingdom3

Received 22 July 2005/ Returned for modification 8 September 2005/ Accepted 13 October 2005

Using the enteropathogenic Escherichia coli (EPEC) genome sequence, we found that EPEC E2348/69 has an lpfABCDE gene cluster homologous (about 60% identical at the protein level) to the Salmonella long polar fimbria (LPF) operon. To determine whether this operon is essential for adherence, the lpfABCDE23 genes were deleted from EPEC strain E2348/69 by allelic exchange. Analysis of the resulting EPEC{Delta}lpfABCDE23 strain showed no change in adherence to HeLa cells or to human intestinal biopsy cells in the in vitro organ culture (IVOC) system compared to the wild type. Sera from volunteers experimentally infected with E2348/69 showed no antibody response to the major subunit protein, LpfA. These results suggested that the lpfE23 gene cluster is not necessary for EPEC adherence and attaching/effacing (A/E) lesion formation on human biopsy samples and is not expressed during human infection. We also identified an lpf gene cluster in Citrobacter rodentium strain ICC168 (lpfcr). A {Delta}lpfAcr mutant of ICC168 retained wild-type adherence and A/E lesion-forming activity on HeLa cells. C3H/HeJ mice were infected with a wild-type C. rodentium strain and its lpfAcr isogenic mutant. Both strains were recovered at high levels in stools, and there were no significant differences between the groups both in terms of the number of CFU/organ (colon and cecum) and in terms of the amount of hyperplasia, as measured by weight. Similar results were observed in a second mouse strain, C57BL/6. These data suggest that in addition to playing no apparent role in EPEC pathogenesis, lpfcr is not required for C. rodentium virulence in either the C3H/HeJ or C57BL/6 mouse model.

* Corresponding author. Mailing address: Center for Vaccine Development, University of Maryland School of Medicine, 685 West Baltimore St., Baltimore, MD 21201. Phone: (410) 706-2344. Fax: (410) 706-0182. E-mail: jkaper{at}umaryland.edu.

Editor: J. B. Bliska

Infection and Immunity, January 2006, p. 265-272, Vol. 74, No. 1
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.1.265-272.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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