This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suar, M.
Right arrow Articles by Hardt, W.-D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suar, M.
Right arrow Articles by Hardt, W.-D.

 Previous Article  |  Next Article 

Infection and Immunity, January 2006, p. 632-644, Vol. 74, No. 1
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.1.632-644.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Virulence of Broad- and Narrow-Host-Range Salmonella enterica Serovars in the Streptomycin-Pretreated Mouse Model

Mrutyunjay Suar,1 Jonathan Jantsch,2 Siegfried Hapfelmeier,1 Marcus Kremer,3 Thomas Stallmach,2 Paul A. Barrow,4 and Wolf-Dietrich Hardt1*

Institute of Microbiology, ETH Hönggerberg, 8093 Zürich, Switzerland,1 Institute of Clinical Pathology, Universitätsspital Zürich, 8091 Zürich, Switzerland,2 Institute of Pathology, Technische Universität München, Ismaninger Strasse 22, D-81675 München, Germany,3 Division of Environmental Microbiology, Institute for Animal Health, Compton Laboratory, Newbury, Berkshire RG20 7NN, United Kingdom4

Received 4 June 2005/ Returned for modification 14 July 2005/ Accepted 9 October 2005

Salmonella enterica subspecies I serovars are common bacterial pathogens causing diseases ranging from enterocolitis to systemic infections. Some serovars are adapted to specific hosts, whereas others have a broad host range. The molecular mechanisms defining the virulence characteristics and the host range of a given S. enterica serovar are unknown. Streptomycin pretreated mice provide a surrogate host model for studying molecular aspects of the intestinal inflammation (colitis) caused by serovar Typhimurium (S. Hapfelmeier and W. D. Hardt, Trends Microbiol. 13:497-503, 2005). Here, we studied whether this animal model is also useful for studying other S. enterica subspecies I serovars. All three tested strains of the broad-host-range serovar Enteritidis (125109, 5496/98, and 832/99) caused pronounced colitis and systemic infection in streptomycin pretreated mice. Different levels of virulence were observed among three tested strains of the host-adapted serovar Dublin (SARB13, SD2229, and SD3246). Several strains of host restricted serovars were also studied. Two serovar Pullorum strains (X3543 and 449/87) caused intermediate levels of colitis. No intestinal inflammation was observed upon infection with three different serovar Paratyphi A strains (SARB42, 2804/96, and 5314/98) and one serovar Gallinarum strain (X3796). A second serovar Gallinarum strain (287/91) was highly virulent and caused severe colitis. This strain awaits future analysis. In conclusion, the streptomycin pretreated mouse model can provide an additional tool to study virulence factors (i.e., those involved in enteropathogenesis) of various S. enterica subspecies I serovars. Five of these strains (125109, 2229, 287/91, 449/87, and SARB42) are subject of Salmonella genome sequencing projects. The streptomycin pretreated mouse model may be useful for testing hypotheses derived from this genomic data.

* Corresponding author. Mailing address: Institute of Microbiology, Department of Biology, ETH Hönggerberg, Wolfgang-Pauli-Str. 10, 8093, Zürich, Switzerland. Phone: 41-44-632-5143. Fax: 41-44-632-1129. E-mail: hardt{at}micro.biol.ethz.ch.

Editor: J. B. Bliska

Infection and Immunity, January 2006, p. 632-644, Vol. 74, No. 1
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.1.632-644.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Suar, M., Periaswamy, B., Songhet, P., Misselwitz, B., Muller, A., Kappeli, R., Kremer, M., Heikenwalder, M., Hardt, W.-D. (2009). Accelerated Type III Secretion System 2-Dependent Enteropathogenesis by a Salmonella enterica Serovar Enteritidis PT4/6 Strain. Infect. Immun. 77: 3569-3577 [Abstract] [Full Text]  
  • Hu, Q., Coburn, B., Deng, W., Li, Y., Shi, X., Lan, Q., Wang, B., Coombes, B. K., Finlay, B. B. (2008). Salmonella enterica Serovar Senftenberg Human Clinical Isolates Lacking SPI-1. J. Clin. Microbiol. 46: 1330-1336 [Abstract] [Full Text]  
  • Raffatellu, M., Santos, R. L., Chessa, D., Wilson, R. P., Winter, S. E., Rossetti, C. A., Lawhon, S. D., Chu, H., Lau, T., Bevins, C. L., Adams, L. G., Baumler, A. J. (2007). The Capsule Encoding the viaB Locus Reduces Interleukin-17 Expression and Mucosal Innate Responses in the Bovine Intestinal Mucosa during Infection with Salmonella enterica Serotype Typhi. Infect. Immun. 75: 4342-4350 [Abstract] [Full Text]  
  • Stecher, B., Paesold, G., Barthel, M., Kremer, M., Jantsch, J., Stallmach, T., Heikenwalder, M., Hardt, W.-D. (2006). Chronic Salmonella enterica Serovar Typhimurium-Induced Colitis and Cholangitis in Streptomycin-Pretreated Nramp1+/+ Mice. Infect. Immun. 74: 5047-5057 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»