This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, S.
Right arrow Articles by Jacobs, W. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, S.
Right arrow Articles by Jacobs, W. R., Jr.

 Previous Article  |  Next Article 

Infection and Immunity, November 2006, p. 6491-6495, Vol. 74, No. 11
0019-9567/06/$08.00+0     doi:10.1128/IAI.00531-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Protection Elicited by Two Glutamine Auxotrophs of Mycobacterium tuberculosis and In Vivo Growth Phenotypes of the Four Unique Glutamine Synthetase Mutants in a Murine Model{dagger}

Sunhee Lee,1 Bo-Young Jeon,2 Svetoslav Bardarov,3 Mei Chen,1 Sheldon L. Morris,2 and William R. Jacobs Jr.1*

Howard Hughes Medical Institute and Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York,1 Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland,2 Department of Pathology, University of Massachusetts, Worcester, Massachusetts3

Received 31 March 2006/ Returned for modification 19 May 2006/ Accepted 6 July 2006

We generated four individual glutamine synthetase (GS) mutants ({Delta}glnA1, {Delta}glnA2, {Delta}glnA3, and {Delta}glnA4) and one triple mutant ({Delta}glnA1EA2) of Mycobacterium tuberculosis to investigate the roles of GS enzymes. Subcutaneous immunization with the {Delta}glnA1EA2 and {Delta}glnA1 glutamine auxotrophic mutants conferred protection on C57BL/6 mice against an aerosol challenge with virulent M. tuberculosis, which was comparable to that provided by Mycobacterium bovis BCG vaccination.

* Corresponding author. Mailing address: Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461. Phone: (718) 430-2865. Fax: (718) 518-0366. E-mail: jacobsw{at}hhmi.org/.

{dagger} Supplemental material for this article may be found at http://iai.asm.org.

Editor: J. L. Flynn

Infection and Immunity, November 2006, p. 6491-6495, Vol. 74, No. 11
0019-9567/06/$08.00+0     doi:10.1128/IAI.00531-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tullius, M. V., Harth, G., Maslesa-Galic, S., Dillon, B. J., Horwitz, M. A. (2008). A Replication-Limited Recombinant Mycobacterium bovis BCG Vaccine against Tuberculosis Designed for Human Immunodeficiency Virus-Positive Persons Is Safer and More Efficacious than BCG. Infect. Immun. 76: 5200-5214 [Abstract] [Full Text]  
  • Carroll, P., Pashley, C. A., Parish, T. (2008). Functional Analysis of GlnE, an Essential Adenylyl Transferase in Mycobacterium tuberculosis. J. Bacteriol. 190: 4894-4902 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»