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Infection and Immunity, February 2006, p. 1436-1441, Vol. 74, No. 2
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.2.1436-1441.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Development of a Guinea Pig Immune Response-Related Microarray and Its Use To Define the Host Response following Mycobacterium bovis BCG Vaccination {dagger}

Julia A. Tree,* Michael J. Elmore, Sajid Javed, Ann Williams, and Philip D. Marsh

Health Protection Agency, Centre for Emergency Preparedness and Response, Porton Down, Salisbury, Wiltshire SP4 0JG, United Kingdom

Received 13 July 2005/ Returned for modification 27 August 2005/ Accepted 11 November 2005

Immune responses in the guinea pig model are understudied because of a lack of commercial reagents. We have developed a custom-made guinea pig oligonucleotide microarray (81 spots) and have examined the gene expression profile of splenocytes restimulated in vitro from Mycobacterium bovis BCG-vaccinated and naive animals. Eleven genes were significantly (P < 0.05) up-regulated following vaccination, indicating a Th1-type response. These results show that microarrays can be used to more fully define immune profiles of guinea pigs.

* Corresponding author. Mailing address: Health Protection Agency, Centre for Emergency Preparedness and Response, Porton Down, Salisbury, Wiltshire SP4 0JG, United Kingdom. Phone: 44 1980 612315. Fax: 44 1980 611096. E-mail: julia.tree{at}hpa.org.uk.

Editor: J. L. Flynn

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Infection and Immunity, February 2006, p. 1436-1441, Vol. 74, No. 2
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.2.1436-1441.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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