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Infection and Immunity, April 2006, p. 2115-2120, Vol. 74, No. 4
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.4.2115-2120.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Genetic Resistance to Brucella abortus in the Water Buffalo (Bubalus bubalis)

Giorgia Borriello,1 Rosanna Capparelli,2 Michele Bianco,3 Domenico Fenizia,4 Flora Alfano,4 Federico Capuano,4 Danilo Ercolini,2 Antonio Parisi,5 Sante Roperto,6 and Domenico Iannelli1*

Chair of Immunology,1 Faculty of Biotechnological Sciences,2 Istituto Zooprofilattico Sperimentale per il Mezzogiorno,4 Istituto Zooprofilattico Sperimentale della Puglia e Basilicata,5 Faculty of Veterinary Medicine, University of Naples "Federico II,",6 Regione Campania, Assessorato all'Agricoltura, Naples, Italy3

Received 30 June 2005/ Returned for modification 25 October 2005/ Accepted 16 January 2006

Brucellosis is a costly disease of water buffaloes (Bubalus bubalis). Latent infections and prolonged incubation of the pathogen limit the efficacy of programs based on the eradication of infected animals. We exploited genetic selection for disease resistance as an approach to the control of water buffalo brucellosis. We tested 231 water buffalo cows for the presence of anti-Brucella abortus antibodies (by the agglutination and complement fixation tests) and the Nramp1 genotype (by PCR-denaturing gradient gel electrophoresis). When the 231 animals (58 cases and 173 controls) were divided into infected (seropositive) and noninfected (seronegative) groups and the Nramp1 genotypes were compared, the seropositive subjects were 52 out of 167 (31%) in the Nramp1A+ (Nramp1AA or Nramp1AB) group and 6 out of 64 (9.4%) in the Nramp1A– (Nramp1BB) group (odds ratio, 4.37; 95% confidence limits, 1.87 to 10.19; {chi}2, 11.65 for 1 degree of freedom). Monocytes from Nramp1BB subjects displayed significantly (P < 0.01) higher levels of Nramp1 mRNA than Nramp1AA subjects and also a significantly (P < 0.01) higher ability in controlling the intracellular replication of several Brucella species in vitro. Thus, selection for the Nramp1BB genotype can become a valuable tool for the control of water buffalo brucellosis in the areas where the disease is endemic.

* Corresponding author. Mailing address: Cattedra di Immunologia, Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Università degli Studi di Napoli "Federico II," Via Università, 133, 80055, Portici, Naples, Italy. Phone: 39-081-2539277. Fax: 39-081-7762886. E-mail: iannelli{at}unina.it.

Editor: V. J. DiRita

Infection and Immunity, April 2006, p. 2115-2120, Vol. 74, No. 4
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.4.2115-2120.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Marianelli, C., Martucciello, A., Tarantino, M., Vecchio, R., Iovane, G., Galiero, G. (2008). Evaluation of Molecular Methods for the Detection of Brucella Species in Water Buffalo Milk. J DAIRY SCI 91: 3779-3786 [Abstract] [Full Text]  
  • Paixao, T. A., Poester, F. P., Carvalho Neta, A. V., Borges, A. M., Lage, A. P., Santos, R. L. (2007). NRAMP1 3' Untranslated Region Polymorphisms Are Not Associated with Natural Resistance to Brucella abortus in Cattle. Infect. Immun. 75: 2493-2499 [Abstract] [Full Text]  
  • Capparelli, R., Alfano, F., Amoroso, M. G., Borriello, G., Fenizia, D., Bianco, A., Roperto, S., Roperto, F., Iannelli, D. (2007). Protective Effect of the Nramp1 BB Genotype against Brucella abortus in the Water Buffalo (Bubalus bubalis). Infect. Immun. 75: 988-996 [Abstract] [Full Text]  


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