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Infection and Immunity, April 2006, p. 2453-2456, Vol. 74, No. 4
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.4.2453-2456.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

RrgA and RrgB Are Components of a Multisubunit Pilus Encoded by the Streptococcus pneumoniae rlrA Pathogenicity Islet

Julianna LeMieux, David L. Hava,{dagger} Alan Basset,{ddagger} and Andrew Camilli*

Howard Hughes Medical Institute and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, Massachusetts 02111

Received 5 November 2005/ Returned for modification 15 November 2005/ Accepted 16 January 2006

The rlrA pathogenicity islet in Streptococcus pneumoniae TIGR4 encodes three surface proteins, RrgA, RrgB, and RrgC, and three sortase enzymes. Using transmission electron microscopy, cell fractionation, cell wall sorting signal domain swapping, and Western blotting, we show that RrgA and RrgB are incorporated into a multisubunit pilus in S. pneumoniae.

* Corresponding author. Mailing address: Howard Hughes Medical Institute and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111. Phone: (617) 636-2144. Fax: (617) 636-2175. E-mail: andrew.camilli{at}tufts.edu.

Editor: V. J. DiRita

{dagger} Present address: Lymphocyte Biology Section, Brigham and Women's Hospital, Harvard Medical School, 1 Jimmy Fund Way, Boston, MA 02115.

{ddagger} Present address: Department of Infectious Disease, Children's Hospital, 300 Longwood Avenue, Boston, MA 02115.

Infection and Immunity, April 2006, p. 2453-2456, Vol. 74, No. 4
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.4.2453-2456.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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