This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Medaglini, D.
Right arrow Articles by Pozzi, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Medaglini, D.
Right arrow Articles by Pozzi, G.

 Previous Article  |  Next Article 

Infection and Immunity, May 2006, p. 2760-2766, Vol. 74, No. 5
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.5.2760-2766.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

In Vivo Activation of Naive CD4+ T Cells in Nasal Mucosa-Associated Lymphoid Tissue following Intranasal Immunization with Recombinant Streptococcus gordonii

Donata Medaglini,1* Annalisa Ciabattini,1 Anna Maria Cuppone,1 Caterina Costa,1 Susanna Ricci,1 Massimo Costalonga,2 and Gianni Pozzi1

Laboratorio di Microbiologia Molecolare e Biotecnologia, Dipartimento di Biologia Molecolare, Università di Siena, 53100 Siena, Italy,1 Department of Developmental and Surgical Sciences, University of Minnesota School of Dentistry, Minneapolis, Minnesota2

Received 14 December 2005/ Returned for modification 30 January 2006/ Accepted 4 February 2006

The antigen-specific primary activation of CD4+ T cells was studied in vivo by adoptive transfer of ovalbumin-specific transgenic T cells (KJ1-26+ CD4+) following intranasal immunization with recombinant Streptococcus gordonii. A strain of S. gordonii expressing on its surface a model vaccine antigen fused to the ovalbumin (OVA) peptide from position 323 to 339 was constructed and used to study the OVA-specific T-cell activation in nasal mucosa-associated lymphoid tissue (NALT), lymph nodes, and spleens of mice immunized by the intranasal route. The recombinant strain, but not the wild type, activated the OVA-specific CD4+ T-cell population in the NALT (89% of KJ1-26+ CD4+ T cells) just 3 days following immunization. In the cervical lymph nodes and in the spleen, the percentage of proliferating cells was initially low, but it reached the peak of activation at day 5 (90%). This antigen-specific clonal expansion of KJ1-26+ CD4+ T cells after intranasal immunization was obtained with live and inactivated recombinant bacteria, and it indicates that the NALT is the site of antigen-specific T-cell priming.

* Corresponding author. Mailing address: LAMMB, Università di Siena, Policlinico Le Scotte, V lotto piano 1, Viale Bracci, 53100 Siena, Italy. Phone: 39 0577 233307. Fax: 39 0577 233334. E-mail: medaglini{at}unisi.it.

Editor: J. T. Barbieri

Infection and Immunity, May 2006, p. 2760-2766, Vol. 74, No. 5
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.5.2760-2766.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ciabattini, A., Pettini, E., Andersen, P., Pozzi, G., Medaglini, D. (2008). Primary Activation of Antigen-Specific Naive CD4+ and CD8+ T Cells following Intranasal Vaccination with Recombinant Bacteria. Infect. Immun. 76: 5817-5825 [Abstract] [Full Text]  
  • Matthias, K. A., Roche, A. M., Standish, A. J., Shchepetov, M., Weiser, J. N. (2008). Neutrophil-Toxin Interactions Promote Antigen Delivery and Mucosal Clearance of Streptococcus pneumoniae. J. Immunol. 180: 6246-6254 [Abstract] [Full Text]  
  • Ciabattini, A., Cuppone, A. M., Pulimeno, R., Iannelli, F., Pozzi, G., Medaglini, D. (2006). Stimulation of Human Monocytes with the Gram-Positive Vaccine Vector Streptococcus gordonii.. CVI 13: 1037-1043 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»