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Infection and Immunity, May 2006, p. 3021-3026, Vol. 74, No. 5
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.5.3021-3026.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Role for RpoS but Not RelA of Legionella pneumophila in Modulation of Phagosome Biogenesis and Adaptation to the Phagosomal Microenvironment

Alaeddin Abu-Zant, Rexford Asare, James E. Graham, and Yousef Abu Kwaik*

Departments of Microbiology and Immunology, University of Louisville College of Medicine, Louisville, Kentucky 40292

Received 5 December 2005/ Returned for modification 22 December 2005/ Accepted 1 February 2006

The induction of virulence traits by Legionella pneumophila at the post-exponential phase has been proposed to be triggered by the stringent response mediated by RelA, which triggers RpoS. We show that L. pneumophila rpoS but not relA is required for early intracellular survival and replication within human monocyte-derived macrophages and Acanthamoeba polyphaga. In addition, L. pneumophila rpoS but not relA is required for expression of the pore-forming activity. We provide evidence that RpoS plays a role in the modulation of phagosome biogenesis and in adaptation to the phagosomal microenvironment. Thus, there is no functional link between the stringent response and RpoS in the pathogenesis of L. pneumophila.

* Corresponding author. Mailing address: Departments of Microbiology and Immunology, University of Louisville College of Medicine, Louisville, KY 40292. Phone: (502) 852-4117. Fax: (502) 852-7531. E-mail: abukwaik{at}louisville.edu.

Editor: J. T. Barbieri

Infection and Immunity, May 2006, p. 3021-3026, Vol. 74, No. 5
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.5.3021-3026.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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