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Infection and Immunity, June 2006, p. 3513-3518, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.00079-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Genomic Diversity between Strains of the Same Serotype and Multilocus Sequence Type among Pneumococcal Clinical Isolates

Nuno A. Silva,1,{dagger} Jackie McCluskey,1,{dagger} Johanna M. C. Jefferies,1,2 Jason Hinds,3 Andrew Smith,4 Stuart C. Clarke,2 Tim J. Mitchell,1* and Gavin K. Paterson1,{ddagger}

Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom,1 Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital, Glasgow, G21 3UW, United Kingdom,2 Department of Medical Microbiology, St. George's Hospital Medical School, Cranmer Terrace, London SW17 0RE, United Kingdom,3 Infection Research Group, Level 9, Glasgow Dental School, University of Glasgow, Glasgow G2 3JZ, United Kingdom4

Received 16 January 2006/ Returned for modification 24 February 2006/ Accepted 27 March 2006

The important human pathogen Streptococcus pneumoniae is known to be a genetically diverse species. We have used comparative genome hybridization (CGH) microarray analysis to investigate this diversity in a collection of clinical isolates including several capsule serotype 14 pneumococci, a dominant serotype among disease isolates. We have identified three new regions of diversity among pneumococcal isolates and, importantly, clearly demonstrate genetic differences between strains of the same multilocus sequence type (ST) and capsule serotype. CGH may therefore, under certain circumstances, prove to be a valuable tool to supplement current typing methods. Finally, we show that these clonal strains with the same serotype and ST behave differently in an animal model. Strains of the same ST and serotype therefore have important genetic and phenotypic differences.

* Corresponding author. Mailing address: Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom. Phone: 44 141 330 3749. Fax: 44 141 330 3727. E-mail: t.mitchell{at}bio.gla.ac.uk.

Editor: J. N. Weiser

{dagger} N.A.S. and J.M. contributed equally to this study.

{ddagger} Present address: Biomedical Sciences, DSTL, Porton Down, Salisbury SP4 0JQ, United Kingdom.

Infection and Immunity, June 2006, p. 3513-3518, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.00079-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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